| 痰热清注射液对急性肝损伤大鼠的保护作用 |
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| 引用本文： | 雷扬,周爱民,郭涛,谭烨,陶艳艳,刘成海. 痰热清注射液对急性肝损伤大鼠的保护作用[J]. 中国中药杂志, 2013, 38(8): 1226-1230 |
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| 作者姓名： | 雷扬 周爱民 郭涛 谭烨 陶艳艳 刘成海 |
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| 作者单位： | 1. 上海中医药大学附属曙光医院肝病研究所,上海,201203
2. 上海中医药大学附属曙光医院肝病研究所,上海201203;上海高校中医内科学E-研究院,上海201203;上海市中医临床重点实验室,上海201203 |
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| 基金项目： | 上海市医学领军人才计划项目(LJ10005);上海高校中医内科学E研究院建设项目(E03008);上海市教委科研创新项目(11YZ63);国家中医药管理局中医肝胆病重点学科、上海高校创新团队建设项目 |
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| 摘 要： | 目的:观察痰热清注射液对四氯化碳(CCl4)诱导大鼠急性肝损伤的保护作用.方法:首先复制CCl4单次染毒模型,100％ CCl45mL·kg-1皮下注射,同时设正常对照组,观察染毒3,6h后大鼠肝损伤变化；继而复制CCl4多次染毒肝损伤模型,首次100％ CCl45 mL·kg-1,第2次50％ CCl4橄榄油溶液2 mL·kg-1,第3次20％ CCl4橄榄油溶液2 mL·kg-1皮下注射诱导急性肝损伤模型,首次CCl4染毒6h后分为模型组、甘草酸二铵治疗组、痰热清高、中、低剂量治疗组,连续尾静脉给药7d,同时设正常对照组.观察大鼠体重、肝体比；HE染色观察肝组织炎症病理；试剂盒检测血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(T.Bil)、直接胆红素(D.Bil)、胆碱酯酶(CHE)、总胆汁酸(TBA)、γ-氨基酰转肽酶(γ-GT)、白蛋白(Alb).结果:单次染毒模型:CCl4单次皮下注射6h后,大鼠血清AST活性与T.Bil含量明显升高,肝组织小叶结构紊乱、肝细胞肿胀明显,呈现明显肝损伤.多次染毒药效实验结果发现:与正常组相比,模型组的ALT,AST与γ-GT活性明显升高(P＜0.05),TBA,T.Bil含量明显增加(P＜0.05),CHE含量明显降低(P＜0.05)；HE染色显示,模型组肝小叶结构排列紊乱,肝细胞气球样变明显.与模型组相比,痰热清高、中剂量组与甘草酸二铵组血清肝功能、肝组织炎症病理明显改善,其中痰热清高、中剂量组优于甘草酸二铵组.结论:痰热清注射液具有显著抗CCl4诱导的大鼠急性肝损伤作用.
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| 关 键 词： | 急性肝损伤 痰热清注射液 四氯化碳 |
| 收稿时间： | 2012-12-31 |
Protective effect of Tanreqing injection on acute hepatic injury induced by CCl4 in rats |
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| LEI Yang,ZHOU Ai-min,GUO Tao,TAN Ye,TAO Yan-yan and LIU Cheng-hai. Protective effect of Tanreqing injection on acute hepatic injury induced by CCl4 in rats[J]. China Journal of Chinese Materia Medica, 2013, 38(8): 1226-1230 |
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| Authors: | LEI Yang ZHOU Ai-min GUO Tao TAN Ye TAO Yan-yan LIU Cheng-hai |
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| Affiliation: | Institute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Institute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Institute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Institute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Institute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Institute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;E-Institute of Traditional Chinese Medicine Internal Medicine, Shanghai Municipal Education Commission, Shanghai 201203, China;Shanghai Key Laboratory of Traditional Chinese Medicine, Shanghai 201203, China |
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| Abstract: | |
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| Keywords: | acute hepatic injury Tanreqing injection CCl4 |
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