三因素条件下AM真菌对半夏侵染率和入药品质的影响

目的:模拟土壤AM真菌关键影响因子,探索AM真菌对半夏根部侵染率和入药质量影响,筛选半夏、AM真菌和土壤环境的最佳组合.方法:设置3因素3水平正交试验,全苗期和成熟期测定半夏AM真菌侵染率,收获期测定半夏块茎水分、水溶性浸出物和入药成分.结果与结论:半夏成熟期90mg.kg-1栽培基质速效磷(P)含量,30％含水量,与EM混合接种时,AM侵染率为最大.各因素对半夏药材水分含量的影响作用大小为菌剂＞P营养＞水分,对半夏水溶性浸出物含量的影响作用大小为水分＞菌剂＞P营养,对半夏块茎琥珀酸和生物碱含量的影响作用大小均为菌剂＞P营养＞水分.30,90mg kg-1的低P条件,接种AM真菌可显著提高AM真菌的侵染率,提高半夏块茎入药质量,但EM和GM混合接种对提高AM真菌侵染率和入药质量的作用上更具有优势性.

Influence on AM fungi infection rate and medicine quality of Pinellia ternata in condition of three soil impact factors

Objective: To explore the influence on AM fungi infection rate and medicine quality of Pinellia ternate in the condition of three soil impact factors. Method: Set the orthogonal test of three factors and levels. Determinate the AM fungi infection rate in early stage of mature & stage of mature of P. ternata, and the water content, water soluble extract, butanedioic acid content and alkaloid content of P. ternata tuber that be harvested also had be determinated. Result and Conclusion: With the P levels to 30 mg·kg^-1 and 90 mg·kg^-1, AM fungi infection was the best when mixed inoculated of EM. Microbial agent inoculated played a decisive role in P. ternata growth and physiological activity, secondary influenced factor was P concentration, and the water stress was the minimal impact. Mixed inoculated of AM fungi and EM treatment with the low P levels(30,90 mg·kg^-1) proved better effect on enhancing the water extract content, anedioic acid and alkaloid content, while decreasing the water contents of P. ternata tuber.