冷冻保存细胞组织的初步研究

就冷冻保存牛关节软骨以及人骨髓成纤维HBMF单层细胞中的有关问题及相关技术进行了初步研究。结果表明:(1)在高浓度的冷冻保护剂CPA中,加入适量的蔗糖小分子并提高1,2-丙二醇含量,可明显限制溶液中冰核形成及结晶生长,有助于提高冷冻保存效果。(2)降低CPA加入、移出的温度,可明显提高软骨细胞的存活率以适当的方式加入、移出CPA后,HBMF单层细胞对高达6.00mol/L的CPA仍可保持较高的存活率。(3)关节软骨直接放在液氮中储存时,组织中的软骨细胞及细胞外基质均产生了严重的破坏而对照组的软骨细胞及细胞外基质具有正常的结构形态。

Study on cryopreservation of cells and tissues

Cryopreservation of bovine articular cartilage and Human Bone Marrow Fibroblast (HBMF) monolayer cells is studied in the paper. The results show that the high concentrations of Cryoprotective agents (CPA) coupled with the appearance of sucrose and high contents of 1,2-propanediol contribute to reducing the formation and growth of ice nuclei and have the potential to obtain good protective effects. High concentrations of CPA can result in great damages to cartilage and HBMF monolayer cells, which can be moderated by decreasing the temperature of adding and removal of CPA. In the frozen cartilage, which is put into liquid nitrogen without adding of any CPA, the architectures of both the chondrocytes and extracellular matrix have been severely damaged. While in the control group, the chondrocytes are intact and well preserved.