大鼠舌下神经切断后舌内肌运动神经元诱向因子及其受体的变化

用本实验室制备的运动神经元诱向因子单克隆抗体和与其受体能特异结合的ＭＮＴＦ抗独特型单抗，对切断一侧舌下神经的３０只大鼠舌内肌，分别在手术后４ｄ，２周，３周和５周以及２个月和５个月，检测其ＭＮＴＦ和ＭＮＴＦ受体定位和含量的变化。从ＬＳＭ ＶＩＤＡＳ图像分析的结果显示，在舌下神经一侧切断４ｄ后，同侧舌内肌ＭＮＴＦ的含量比同体对侧未切断舌下神经的舌内肌含量减至约７５％～８０％，但此时期同侧舌内肌ＭＮＴＦ

CHANGES IN MOTO-NEURONOTROPHIC FACTOR AND ITS RECEPTOR IN TONGUE MUSCLE POST-DENERVATION OF THE HYPOGLOSSAL NERVE

We have used our anti-MNTF monoclonal antibody (MNTF-McAb) and MNTF-anti- idiotypic monoclonal antibody (MNTF-Id-McAb) to study the changes in localization and quantities of MNTF and its receptor in tongue muscles of 30 adult rats after 4 days, 2, 3 and 5 weeks, and 2 and 5 months of post-denervation of the hypoglossal nerve. The LSM VIDAS image analysis results revealed that 4 days post-denervation, the MNTF content in tongue muscles of the denervated side had reduced to 75%-80%as compared to the control intact side 100%, however, the reduction of MNTF receptor was insignificant; 2-3 weeks post- denervation, the contents of MNTF and its receptor in the denervated side were significantly reduced to 65%-70% and 50%-55%, respectively; 5 weeks post- denervation, the contents of MNTF and its receptor had started to increase especially for the receptor; and gradually the contents of both MNTF and its receptor in denervated side had recovered and reached to a similar levels as in the control side at 2 months and 5 months post-denervation. These results indicated that MNTF and its receptor are synthesized by the tongue muscle cells and their syntheses are regulated by the hypoglossal nerve; and that there is a close relationship between MNTF and its receptor with the regeneration of hypoglossal nerve.