细胞趋化因子受体5在病毒性肝炎患者外周血单核细胞中的表达

目的研究细胞趋化因子受体5（CCR5）在乙型病毒性肝炎（HB）免疫发病机制中的作用。方法用流式细胞仪对20例正常人（NC）及20例HB患者外周血单核细胞（PBMC）水平进行检测，并比较其经植物凝血素（PHA）培养前后的变化。结果培养前NC与HB患者PBMC的CCR5的表达无明显差异（P〉0．05）；培养后HB患者CCR5表达水平高于NC（P〈0．05）；NC的CCR5表达均较培养前明显减少（P〈0．05），而HB患者的CCR5表达虽较培养前减少，但无统计学意义（P〉0．05）；急性乙型肝炎（AHB）、慢性乙型肝炎（CHB）、肝硬化（LC）患者的CCR5的表达有一定的差别：AHB〉CHB〉LC，但无统计学意义（P〉0．05）。病毒复制指标阳性组CCR5的表达明显低于阴性组（P〈0．05）。结论HB患者PBMC的CCR5的表达水平与HB的临床转归可能有一定的关系，高水平的CCR5患者预后较好；PMBC高水平CCR5的表达可能不利于乙型肝炎病毒（HBV）的复制。

CCR5 expression in peripheral blood mononuclear cells of patients with hepatitis B

Objective To investigate the role of CC chemokine receptor 5(CCR5) in the immunological pathogenesis of hepatitis B(HB).?Methods The expression levels of CCR5 in peripheral blood mononuclear cell(PBMC) were assayed by flow cytometry before and after PHA-induced cultivation in 20 HB patients and 20 normal controls(NC).?Results Before cultivation,there was no discrepancy in CCR5 level between NC and HB(P>0.05).After cultivation,CCR5 was obviously lowered in NC group(P<0.05),but insignificantly in HB group(P>0.05),with the slightly lowering CCR5 levels in an order as AHB>CHB>LC.It was also noticed that the CCR5 level was lower in the HB patients positive for HBV duplicating markers than that in those who were negative(P<0.05).?Conclusion CCR5 expression level in PMBC may be related to the outcome of HB,and higher levels of CCR5 suggest relatively favorable prognosis and limit HBV replication.