芦笋多糖对S180小鼠红细胞氯离子浓度及膜电位影响的研究

 目的研究芦笋多糖对S₁₈₀小鼠红细胞膜带3蛋白Cl^-转运功能的影响及其机制。方法采用荧光分光光度法动态观察红细胞内Cl^-的含量;应用流式细胞仪测定红细胞膜电位大小。结果在Cl^-转运功能实验中,模型对照组在20,35min的红细胞荧光强度均明显低于正常组,芦笋多糖3个剂量组红细胞荧光强度较模型对照组显著升高,低、中剂量组作用非常显著(P<0.01),高剂量组在35min也有显著作用(P<0.05);在膜电位实验中,模型对照组S₁₈₀小鼠荧光强度(45.0%)较正常小鼠(99.4%)显著降低(P<0.01),而低、中、高剂量芦笋多糖能使S₁₈₀小鼠红细胞膜电位显著升高(P<0.01)。结论芦笋多糖能够显著增强红细胞膜的Cl^-转运的功能,并且芦笋多糖的这一作用可能是通过升高红细胞膜电位而实现的。

Effect of Asparagus Polysaccharide on Cl-Concentration and Membrane Potential in S180 Mice Erythrocytes

OBJECTIVE To observe the influence of Asparagus polysaccharide on Cl^-transportation function of band 3 protein on the erythrocyte membrane of S₁₈₀ mice and its mechanism.METHODS Cl^-transportation function was explored by the fluorescence spectrophotometric method,and Flow Cytometery(FCM)was used to examine the erythrocyte membrane potential.RESULTS In the examination of Cl^-transportation,the fluorescence intensity of negative control group was remarkably lower than that of the normal group,and the fluorescence intensity of polysaccharide groups was higher than that of negative control group,the low dose group and the medium dose group(P<0.01),the high dose group(P<0.05)at 35 min after reaction.In the experiment of membrane potential,the fluorescence intensity of negative group(45.0%)was remarkably lower than that of the normal group(99.4%),and the fluorescence intensity of polysaccharide groups(65.5%,98.4%,89.9%)was higher than that of negative group.CONCLUSION Asparagus polysaccharide obviously enhanced the Cl^-transportation function of erythrocyte band 3 of S₁₈₀ mice,and the action was obtained possibly through elevating erythrocyte membrane potential.