| 环介导恒温扩增技术在轮状病毒基因检测中的应用 |
| |
| 引用本文： | 王滔,陈宗峰,陈骏扬,黄祖新. 环介导恒温扩增技术在轮状病毒基因检测中的应用[J]. 福建医药杂志, 2010, 32(6): 6-8 |
| |
| 作者姓名： | 王滔 陈宗峰 陈骏扬 黄祖新 |
| |
| 作者单位： | [1]福建省立医院福建省儿科研究室,福州350001 [2]福建师范大学生命科学院,福州350001 |
| |
| 基金项目： | 福建省科技计划资助项目 |
| |
| 摘 要： | 目的建立环介导恒温扩增技术快速检测轮状病毒的方法。方法采用环介导恒温扩增反应（LAMP）技术扩增轮状病毒特异性基因,并与传统PCR技术检测结果比较。结果 LAMP法更加简便快速、且在恒温条件下进行,特异性好,且具有更高的灵敏性。结论 LAMP方法具有灵敏度高、特异性强、操作简便快速、不需要复杂仪器设备等优点,为临床检测轮状病毒提供了快速简便的新方法 。
|
| 关 键 词： | 轮状病毒 环介导恒温扩增 基因检测 |
Detection of rotavirus gene by loop-mediated isothermal amplification |
| |
| WANG Tao,CHEN Zong-feng,CHEN Jun-yang,HUANG Zu-xin. Detection of rotavirus gene by loop-mediated isothermal amplification[J]. Fujian Medical Journal, 2010, 32(6): 6-8 |
| |
| Authors: | WANG Tao CHEN Zong-feng CHEN Jun-yang HUANG Zu-xin |
| |
| Affiliation: | .Department of Pediatrics,Fujian Provincial Hospital,Fuzhou 350001,China |
| |
| Abstract: | Objective To detect specific gene for rotavirus in clinic samples by loop-mediated isothermal amplification（LAMP）.Methods The specific gene of rotavirus was amplified by LAMP,and compared with the detective result by PCR.Results The LAMP assay could be performed under isothermal conditions,its specificity was good and the sensitivity was higher than that of PCR.Conclusion The LAMP assay is simple,rapid,sensitive and specific,and no special apparatus is needed.These results indicate that the LAMP assay can provide a rapid and simple test for the detection of rotavirus. |
| |
| Keywords: | rotavirus loop-mediated isothermal amplification gene detection |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
