凉润通络方对糖尿病大鼠胃肠MOT、CCK、SS内分泌细胞的影响

目的采用链脲佐菌素(STZ)注射制作糖尿病大鼠模型,探讨凉润通络方对糖尿病大鼠胃肠功能改善的机制。方法雄性Wistar大鼠,采用STZ注射制作糖尿病模型,造模成功后,不予降糖药,正常饲养6周后,大鼠分别予温水、凉润通络中药、西沙必利灌胃,灌胃6周后分别检测血浆胃动素(MOT)、胆囊收缩素(CCK)、生长抑素(SS)激素水平并观察组织的MOT、CCK、SS内分泌细胞变化。结果12周时糖尿病大鼠存在胃肠激素的紊乱,凉润通络方同步影响血液、组织中胃肠激素的分泌和释放,作用靶点较宽,优于西沙必利。结论凉润通络方在改善糖尿病胃肠功能障碍方面具有优势。

Effect of Liangruntongluo Recipe on endocrine cells of MOT, CCK and SS in stomach intestine of rats with diabetes

Objective To study the mechanism of Liangruntongluo Recipe in improving gastrointestinal function of diabetes mellitus (DM) rats induced by streptozocin (STZ).Methods Forty male Wistar rats were divided into four groups randomly, group A as model control group, group B as Liangruntongluo group, group C as Cisapride group, and group D as normal control group. There were 10 rats in each group. After the establishment of DM animal model with Wistar rats, there were not any therapy were adopted. After six weeks, medicines were administrated intragastrically for 6 weeks, then plasma motilin (MOT), cholecystokinin (CCK) and somatostatin (SS) were detected and endocrine cells of MOT, CCK and SS in gastrointestinal tissues were observed.Results By the time of the 12th week, the rats with DM presented the disorder of gastrointestinal hormone. Liangruntongluo Recipe affected the secretion and releasing of hormone in blood and gastrointestinal tissues. The target range was wider and was superior to Cisapride.Conclusions The Chinese medicine Liangruntongluo Recipe has the superiority in improving the disturbance of the gastrointestinal function of diabetes mellitus.