β-细辛醚对小鼠脑组织基因表达谱的影响

目的：应用基因芯片技术，观察β-细辛醚对βALβ／c小鼠脑组织基因表达谱的影响。方法：将β-细辛醚用药组与空白对照组小鼠脑组织的mRNA分别用cy5、cy3荧光标记，混合后与4000条基因杂交于MGEC-40S小鼠表达谱芯片上，重复制作二张片,Genepix400B扫描仪扫描杂交信号荧光强度，GenepixPro3．0图象处理软件分析扫描结果。结果：在4000条基因中，二张片中两组都出现差异表达且比值大于1．9或小于0．59的基因共15条。β-细辛醚组有4条基因表达上调，11条基因表达下调。表达上调的4条基因主要与离子通道、细胞的跨膜物质变换、钙依赖性蛋白激酶调节、减少细胞凋亡等功能有关。而表达下调的11条基因主要与脑内兴奋性氨基酸的代谢、T淋巴细胞的趋化作用、细胞基因表达调控、药物代谢等功能有关。结论：β-细辛醚对脑组织多个靶基因有作用。

Effects of β-asaron on Gene Expression in Mouse Brain

OBJECTIVE: To observe effects of beta-asaron on gene expression in BALB/c mouse brain by gene chip. METHODS: The mRNA of mouse brain samples of experiment group and control group was markered with cy5, cy3 fluorescence separately, and hybridized with 4000 genes on MGEC-40S gene chip after mix. Fluorescence intensity was scanned with Genepix 400 B scanner and analysed with Genepix Pro3.0 soft. RESULTS: Among 4000 genes, there are 15 genes with difference expression (the ratio > 1.9 or < 0.59) in two groups. In beta-asaron group, 4 genes expression raised relating to ion channels, substance transform through membrane, calcium-dependent protein kinase regulation, apoptosis, 11 genes expression declined relating to excitatory neurotransmitter metabolism in the CNS, T lymphocyte tend, genes expression regulation and drug metabolism. CONCLUSION: beta-asaron has effects on multi-target genes in mouse brain.