山莨菪碱在大鼠脑缺血再灌注时对神经元调亡的影响

目的：通过观察脑缺血再灌注后海马CAI区存活神经元数目、原位末端标记（TUNEL）阳性细胞数目、热休克蛋白70（HSP70）阳性细胞数目以及脑组织超微结构的变化，探讨山莨菪碱（Anisodamine,Ani）对脑缺血再灌注损伤保护作用的机制。方法：采用Pulsinelli法建立大鼠全脑缺血再灌注损伤模型。观察Ani在脑缺血再灌注的迟发性损伤阶段对海马CAI区存活神经元数目、TUNEL阳性细胞数目、HSP70阳性细胞数目以及脑组织超微结构的影响。结果：Ani可增加脑缺血再灌注后的HSP70的表达，减少神经元调亡的发生，提高神经元的存活数目。结论：Ani可减少脑缺血再灌注神经元调亡，提高神经元的存活数目，对脑缺血再灌注损伤起保护作用。

Influence of Anisodamine on neuronal apoptosts after cerebral ischemia reperfusion in the rats

Objective The number of suviving neuron, TUNEL positive neuron, HSPT0 positive neuron and the ultrastructure of the CA1 subfield of hippocampus was detected after the cerebral ischemia repeffusion of rats and the protective effects of anisodamine(Ani) were also observed. Methods Pulsinelli' s method was employed to establish cerebral ischemia - reperfusion animal models. Observing the influence of Ani on the number of surviving neuron,TUNEL positive neuron, HSP70 positive neuron and ultrastructure of the CA1 subfield of hippocampus. Results Ani could induce upregulation of HSP70 expression after cerebral ischemia reperfusion. Neuronal apoptosis after cerebral ischemia reperfusion was decreased and the surviving number of pyramidal celIs in hippocampus CA1 was increased by Ani. Conclusion Ani have a remarkable protection on cerebral ischemia reperfusion damage. Its mechanism may be related with decreasing the neuronal apoptosis.