A high-throughput test to detect C.E.R.A. doping in blood |
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| Authors: | S verine Lamon, Sylvain Giraud, L onie Egli, Jessica Smolander, Michael Jarsch, Kay-Gunnar Stubenrauch, Alice Hellwig, Martial Saugy,Neil Robinson |
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| Affiliation: | aLaboratoire Suisse d’Analyse du Dopage, Centre Universitaire Romand de Médecine Légale, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Croisettes 22, 1066 Epalinges, Switzerland;bService de Néphrologie et d’Hypertension, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland;cPharma Research, Roche Diagnostics GmbH, Penzberg, Germany;dMicroCoat Biotechnologie GmbH, Bernried, Germany |
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| Abstract: | C.E.R.A., a continuous erythropoietin receptor activator, is a new third-generation erythropoiesis-stimulating agent (ESA) that has recently been linked with abuse in endurance sports. In order to combat this new form of doping, we examined an enzyme-linked immunosorbent assay (ELISA) designed to detect the presence of C.E.R.A. in serum samples. The performance of the assay was evaluated using a pilot excretion study that involved six subjects receiving C.E.R.A. Validation data demonstrated an excellent reproducibility and ensured the applicability of the assay for anti-doping purposes. To maximize the chances of detecting the drug in serum samples, we propose the use of this specific ELISA test as a high-throughput screening method, combined with a classic isoelectric focusing test as a confirmatory assay. This strategy should make C.E.R.A. abuse relatively easy to detect, thereby preventing the future use of this drug as a doping agent. |
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| Keywords: | Erythropoietin Blood doping C.E.R.A. ELISA |
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