~（125）I-脱氧尿嘧啶核苷对肺癌细胞A549的杀伤作用

目的探讨125I-脱氧尿嘧啶核苷（125I-UdR）对体外培养的A549肺癌细胞的杀伤作用及其影响因素。方法测定A549肺癌细胞在含有不同浓度125I-UdR的RPMI 1640培养液中培养48 h后细胞及细胞核摄取125I-UdR的量;测定肺癌细胞A549及脐静脉内皮细胞（ECV304）在含有100 kBq/ml 125I-UdR的RPMI 1640培养液中培养2～48 h后不同时间细胞摄取125 I-UdR的量;用细胞克隆形成法评价不同浓度125 I-UdR对A549肺癌细胞的杀伤作用。结果 A549细胞和细胞核摄取125I-UdR的量随培养基中125I-UdR浓度增加而增加;在含有100 kBq/ml浓度125 I-UdR的培养液中,随培养时间的延长A549细胞和细胞核摄取125 I-UdR的量增加。在不同培养时间下,A549细胞摄取125I-UdR的量在各时间点均明显高于ECV304细胞的摄取量。细胞存活分数随培养基中125I-UdR浓度增加呈递减趋势。结论 125I-UdR能够被A549肺癌细胞摄取,并快速进入细胞核内,且摄取量存在浓度依赖性和时间依赖性1,25I-UdR对肺癌细胞具有明显的杀伤作用。

Killing effect of ~(125)I-UdR on lung cancer cell line A549

Objective To investigate the killing effect of 5-iodo-2′-deoxyuridine（125I-UdR） on lung cancer cell line A549 and the factors of influencing in vitro.Methods The amount of 125I-UdR taken by the cells and caryons were measured through counting the radioactivity after incubating for 48 hours in RPMI 1640 culturing medium containing different concentration of 125I-UdR.The amount of 125I-UdR taken by A549 and ECV304 cells were measured in 100 kBq/ml dosage of 125I-UdR after incubating different time（2-48 hours）.The killing effect of 125I-UdR on A549 cell was evaluated by colony forming method.Results The amount of 125I-UdR taken by the cells and karyons increased with the increasing of 125I-UdR concentration.The concentration that A549 and ECV304 cells ingested 125I-UdR was time-dependent among 2-48 hours.The radioactivity concentration of 125I-UdR uptook in A549 cell was evidently higher than that in ECV304 cell in different incubation time.The surviving fraction was gradually decreased with the increasing of 125I-UdR concentration in culturing medium.Conclusions 125I-UdR could be ingested by A549 cell in culturing medium,which showed strong killing effect on the cells.The concentration of 125I-UdR ingestion was influenced by the dose and culturing time.