| 天然药物IHA-01筛选敏感肿瘤细胞株及其对结肠癌细胞增殖的影响 |
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| 引用本文： | 李秋恬,洪敏,陈晓,李少遊,缪延栋,耿计伟,李臣,尹燕鹰,董坚.天然药物IHA-01筛选敏感肿瘤细胞株及其对结肠癌细胞增殖的影响[J].山东医药,2011,51(39):22-24. |
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| 作者姓名： | 李秋恬 洪敏 陈晓 李少遊 缪延栋 耿计伟 李臣 尹燕鹰 董坚 |
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| 作者单位： | 昆明医学院第一附属医院,昆明,650032 |
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| 基金项目： | 云南省科技计划重点新产品开发计划项目(2007BC002),云南省科技计划条件平台建设项目(2007DA006) |
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| 摘 要： | 目的筛选出对天然药物IHA-01的敏感肿瘤细胞,并对其抗瘤机制进行初步研究。方法体外培养12种人肿瘤细胞株,经3.125、6.25、12.5、25和50μg/ml 5个浓度IHA-01作用48 h后光镜下观察细胞株形态变化,采用CCK-8法计算IC50值（半数抑制浓度）,确认敏感细胞株及最佳作用浓度。流式细胞仪检测IHA-01最佳作用浓度下敏感细胞株细胞凋亡情况及端粒酶活性。结果 5个浓度IHA-01均能抑制12种癌细胞增殖,确定结肠癌HCT-8细胞为敏感细胞（IC50值最低）,最佳作用浓度为1.29μg/ml;鼻咽癌CNE-2细胞为不敏感细胞（IC50值最高）。1.29μg/ml的IHA-01作用后HCT-8细胞凋亡率明显高于、端粒酶活性明显低于CNE-2细胞（P均〈0.01）。结论 IHA-01对HCT-8细胞有较强的抑制作用;其机制可能为诱导肿瘤细胞凋亡及抑制细胞端粒酶活性。
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| 关 键 词： | IHA-01 结肠癌细胞 细胞凋亡 端粒酶活性 |
Screening of sensitive tumor cells of traditional Chinese medicine IHA-01 and its effects on the proliferation of colon carcinoma cells |
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| LI Qiu-tian,HONG Min,CHEN Xiao,LI Shao-you,MIAO Yan-dong,GENG Ji-wei,LI Chen,YIN Yan-ying,DONG Jian.Screening of sensitive tumor cells of traditional Chinese medicine IHA-01 and its effects on the proliferation of colon carcinoma cells[J].Shandong Medical Journal,2011,51(39):22-24. |
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| Authors: | LI Qiu-tian HONG Min CHEN Xiao LI Shao-you MIAO Yan-dong GENG Ji-wei LI Chen YIN Yan-ying DONG Jian |
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| Institution: | LI Qiu-tian,HONG Min,CHEN Xiao,LI Shao-you,MIAO Yan-dong,GENG Ji-wei,LI Chen,YIN Yan-ying,DONG Jian(The First Affiliated Hospital of Kunming Medical College,Kunming 650032,P.R.China) |
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| Abstract: | Objective To screen out sensitive tumor cell lines with traditional Chinese medicine IHA-01,and study its anti-tumor mechanism.Methods Twelve strains of human cancer cell lines were cultured in vitro,then treated with IHA-01 at 5 different concentrations for 48 hours.Cell morphology changes were observed by microscope,and IC50(half inhibitory concentration) values was obtained by CCK-8 assay to confirm the sensitive cell line;the apoptosis and telomerase activitieof HCT-8 exposed to the optimal concentratio... |
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| Keywords: | IHA-01 colon carcinoma cells apoptosis telomerase activity |
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