新型PH-20疫苗不同注射方式对雌性豚鼠免疫应答和避孕效果的影响

目的观察以等比方法配制的新型精子表面特异性透明质酸酶（PH-20）疫苗的不同注射方式对豚鼠免疫应答和避孕的影响。方法60只雌性豚鼠随机分为两大组（直肠注射组和腹腔注射组），每组再分为3个小组（实验1组、实验2组和对照组，每小组各10只），用电镜观察该新型PH一20疫苗的形态，应用双夹心酶联免疫吸附试验（ELISA）检测豚鼠阴道分泌物中分泌型免疫球蛋白A（sIgA）的水平、放射免疫分析法检测血液slgA的水平、竞争ELISA技术检测血液PH-20特异性抗体的水平。结果直肠注射组和腹腔注射组豚鼠血液和阴道分泌物中sIgA、PH-20抗体含量均较对照组升高（P均〈0．05）；实验1组（添加弗氏完全佐剂）豚鼠在各测量时间点血液和阴道分泌物中sIgA、PH-20抗体含量均较实验2组（未添加弗氏完全佐剂）高（P均〈0．05）。直肠注射组（50％）和腹腔注射组（76．7％）总阳性怀孕率相比有显著性差异（P〈0．05）。结论该新型PH-20疫苗具有较高的免疫活性，能诱导机体产生明显的体液反应，且直肠注射诱导免疫优于腹腔注射。用弗氏完全佐剂均能增强两种注射方式的避孕效果。

Immune response and effect of contraception in female guinea pigs injected with PH-20 coupling body

Objective To observe the immune response and contraceptive effect of guinea pigs injected with the PH-20 coupling body by different method of injection.Methods Sixty female guinea pigs were divided randomly into two groups(rectal injection group and intraperitoneal injection group),then each group was subdivided into three groups(experimental group 1,experimental group 2 and control group,10 female guinea pigs in each group).To observe morphology of the coupling by electron microscopy,to test vaginal secret...