藤梨根乙酸乙酯提取物对肺癌A549细胞凋亡的诱导作用

目的观察藤梨根乙酸乙酯提取物对肺癌A549细胞凋亡的诱导作用。方法体外培养肺癌A549细胞,分别给予40、80、160μg/ml的藤梨根乙酸乙酯提取物（实验组）,同时设对照组（0.04%DMSO＋肺癌A549细胞）。采用DNA琼脂糖凝胶电泳检测用药后DNA梯状条带、TUNEL检测用药后肺癌A549细胞凋亡率的变化、免疫组化SP法检测用药后肺癌A549细胞Survivin蛋白表达变化、RT-PCR法检测用药后A549细胞Survivin mRNA的表达。结果实验组细胞DNA凝胶电泳均出现凋亡细胞所特有的DNA梯状条带图谱,而对照组细胞未出现。各实验组细胞随着藤梨根乙酸乙酯提取物浓度的增加,细胞凋亡率明显增加,并存在时相性和量—效依赖性;其Survivin蛋白和mRNA表达均低于对照组（P均〈0.05）,亦存在时相性和量—效依赖性。结论藤梨根乙酸乙酯提取物可明显诱导肺癌A549细胞凋亡,其机制可能与降低Survivin蛋白和mRNA表达有关。

Action mechanism of Radix Actinidiae extracts on apoptosis of lung cancer cell line A549

Objective To observe the induced effect of Radix Actinidiae extracts on apoptosis of A549 cell.Methods A549 cells were cultured in vitro,and were given different concentrations of（40 μg/ml,80 μg/ml,160 μg/ml） Radix Actinidiae extracts（treatment group） or 0.04% DMSO（control group）.DNA agarose gel electrophoresis（AGE） was used to detect DNA ladder band of A549 cell after treatment.Cell apoptosis was measured by TUNEL after treatment.Immunohisto chemistry and RT-PCR were used to detect the expressions of Survivin protein and mRNA.Results DNA ladder strap atlas were found in treatment group by DNA agarose gel electrophoresis.Apoptosis rate of A549 cell in each treatment group obviously increased by dose-and time-dependent manner.The expresses of Survivin protein and mRNA in treatment groups were lower than those in control group（P〈0.05）.Conclusions Radix Actinidiae extracts can induce apoptosis of A549 cell in vitro,its mechanism may be related with regulating the expression of Survivin gene.