| VEGF-A对人肝癌细胞体外形成血管生成拟态能力的影响 |
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| 引用本文： | 李蕊,孙保存,孙丹,刘铁菊,赵秀兰,董学易. VEGF-A对人肝癌细胞体外形成血管生成拟态能力的影响[J]. 山东医药, 2011, 51(45): 1-3 |
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| 作者姓名： | 李蕊 孙保存 孙丹 刘铁菊 赵秀兰 董学易 |
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| 作者单位： | 1. 天津医科大学病理学教研室,天津,300070
2. 天津医科大学病理学教研室,天津300070;天津医科大学肿瘤医院 |
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| 基金项目： | 国家自然科学基金项目(30830049); 中瑞国际合作项目(09ZCZDSF04400); 天津市科技计划项目(09JCYBJC12100) |
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| 摘 要： | 目的探讨血管内皮生长因子（VEGF）对人肝癌细胞体外形成血管生成拟态（VM）能力的影响。方法采用不同浓度的VEGF-A诱导人肝癌PLC细胞,分别用划痕实验、明胶酶谱法分析不同浓度VEGF-A对PLC细胞迁移性和侵袭性的影响;三维培养观察VEGF-A对PLC细胞形成VM能力的影响;Western blot法检测VEGF-A处理后的血管内皮钙黏附素（VE-cadherin）表达。结果 VEGF-A呈剂量依赖性增强肝癌PLC细胞的迁移率和侵袭相关蛋白MMP-9的活性,促进管道样结构形成及VE-cadherin表达（P均〈0.05）。VEGF-A水平与管道数量呈正相关（r=0.929,P〈0.05）。结论 VEGF-A可增强肝癌PLC细胞的迁移和侵袭能力,促进其VM形成,使肿瘤组织血供丰富,促进肿瘤浸润和转移。
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| 关 键 词： | 肝肿瘤 血管内皮生长因子 血管生成拟态 明胶酶类 |
Effects of VEGF-A on vasculogenic mimicry in human hepatocellular carcinoma cells in vitro |
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| LI Rui,SUN Bao-cun,SUN Dan,HU Tie-jü,ZHAO Xiu-lan,DONG Xue-yi. Effects of VEGF-A on vasculogenic mimicry in human hepatocellular carcinoma cells in vitro[J]. Shandong Medical Journal, 2011, 51(45): 1-3 |
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| Authors: | LI Rui SUN Bao-cun SUN Dan HU Tie-jü ZHAO Xiu-lan DONG Xue-yi |
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| Affiliation: | LI Rui1,SUN Bao-cun,SUN Dan,LIU Tie-jü,ZHAO Xiu-lan,DONG Xue-yi(1 Department of Pathology,Tianjin Medical University,Tianjin 300070,P.R.China) |
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| Abstract: | Objective To study the effects of vascular endothelial growth factor(VEGF) on vasculogenic mimicry(VM) of human hepatocellular carcinoma cells in vitro.Methods Hepatocellular carcinoma cells PLC were induced with various concentrations of VEGF-A.The migration and invasion abilities of PLC when treated with various concentrations of VEGF-A were detected by wound-healing and Gelatin zymography assay.The effects of VEGF-A on vasculogenic mimicry in PLC cells were detected in three-dimensional cultures,and the ... |
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| Keywords: | hepatic neoplasm vascular endothelial growth factor vasculogenic mimicry gelatinases |
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