原位杂交测肿瘤坏死因子mRNA在宫颈细胞及子宫内膜组织的定位表达

目的 观察肿瘤坏死因子（mRNA）在宫颈细胞及子宫内膜组织的表达及其在不同病变组织中的分布特点。方法 采用非同位素原位杂交法对3个传代的宫颈细胞系（HiSa、W12、NCx），石蜡包埋的宫颈组织96例、子宫内膜组织75例进行了TNFmRNA写位表达的研究。结果 TNFαmRNA和W12细胞中有阳性表达，NCx细胞未见阳性表达；在宫颈组织中阳性表达见于上皮、间质、血管壁及平滑肌；子宫内膜癌组织中阳性表达见于间质单个核细胞和平滑肌细胞，对照组未见阳性表达。结果 宫颈尖脱湿疣及宫颈鳞癌来源的上皮为TNF生成细胞之一;TNFmRNA表达强度与细胞增生程度有关；宫颈HPV感染对细胞内TNFmRNA表达有促进作用。

Detection of tumor necrosis factor mRNA expression in human cervix and endometrium by in situ hybridization.]

OBJECTIVE: To investigate the expression and distributions of tumor necrosis factor (TNF) mRNA in cells derived from human cervix and endometrium with different pathological changes. METHODS: Non-isotopic in situ hybridization was used to study the locus expression of TNF mRNA in 3 cell lines (SiHa, W12 and NCx) derived from human cervix and 96 paraffin-embedded cervical specimens along with that in 75 specimens of emdometrial tissues. RESULTS: TNF mRNA expression was detected in SiHa and W12 cell lines but not in NCx cells. The cervical specimens under examination presented TNF mRNA expression in the cells from the epithelium, interstitial tissues, vascular walls and smooth muscles. The mononuclear cells and smooth muscle cells of endometrial carcinoma tissues, but not those of the normal endometrial tissues, were positive for TNF mRNA expression. CONCLUSION: Epithelial cells derived from condylomatosis and squamous cell carcinoma of the cervix are one type of TNF-producing cells, and the intensity of TNF mRNA expression is related to the degree of uterine hyperplasia. Human papilloma virus infection may enhance TNF mRNA expression in the cervical cells.