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Effect of poplar-type propolis on oxidative stability and rheological properties of O/W emulsions
Authors:Soukaina El-Guendouz  Smail Aazza  Badiaa Lyoussi  Nessrine Majdoub  Vassya Bankova  Milena Popova  Sara Raposo  Maria Dulce Antunes  Maria Graça Miguel
Affiliation:1. Laboratory of Physiology-Pharmacology-Environmental Health, Faculty of Sciences Dhar El Mehraz, BP 1796 Atlas, University Sidi Mohamed Ben Abdallah, Fez 30 000, Morocco;2. Department of Chemistry and Pharmacy, MeditBio, Faculty of Science and Technology, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal;3. Institute of Organic Chemistry with Centre of Phytochemistry, Acad. G. Bonchev strl. bl. 9, 1113 Sofia, Bulgaria;4. Centre for Marine and Environmental Research (CIMA), Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal;5. CEOT, MeditBio, Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal
Abstract:Propolis is known to possess antioxidant activity. However, there is no information on this activity in emulsions O/W. The protective effect of propolis on the oxidation and rheological properties of emulsions O/W containing wheat germ and almond oils was evaluated. Emulsions O/W were prepared with different concentration of propolis extract, almond oil and wheat germ oil. All emulsions physically stable without phase separation were stored at 37?°C for 9?weeks. Chemical composition of propolis was established by Gas chromatography coupled to mass spectrometry. Rheological characterization of different emulsions was performed evaluating consistency index and flow behavior index. The oxidation was monitored by measuring the lipid hydroperoxides and thiobarbituric acid-reactive substances (TBARS) methods. Flavonoids, phenolic acid esters, and aromatic acids were the main groups of compounds found in propolis. The results showed that popolis was good antioxidant in the concentration of 0.02 and 0.04% when lipid phase was constituted by almond oil. The rheological behavior is typical of a non-Newtonian fluid, being almond oil more adequate for having a higher stable O/W emulsion.
Keywords:Emulsion  Flavonoids  Phenolic acid esters  Almond oil  Wheat germ oil  Natural antioxidant
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