增生性瘢痕和瘢痕疙瘩组织中细胞凋亡与增殖的研究

应用末端脱氧核苷酰转移酶介导的ｄ－ＵＴＰ生物素缺口末端标记技术和免疫组化技术原位检测增生性瘢痕和瘢痕疙瘩组织中的凋亡细胞和增殖细胞核抗原的表达。结果表明：在上述瘢痕组织中，表皮基底层下细胞ＰＣＮＡ阳性评分明显升高，而凋亡指数与正常皮肤相比无明显差异。

Detection of apoptosis by in situ labeling and study on the expression of PCNA in hypertrophic scars and keloids]

To investigate the involvement of apoptosis and proliferation in the lesions of hypertrophic scars and keloids, eighteen samples of hypertrophic scar and five samples of keloid were collected. The apoptosis was detected with terminal deoxynucleatidyl transferase mediated d-UTP biotin nick end labeling(TUNEL) and the expression of PCNA was assessed with immunohistochemical technique in scar lesions and normal skin. Results showed that in hypertrophic scar and keloid samples the rate of proliferating cells in dermis was higher than that of normal skin(P < 0.05), whereas the apoptosis index between scars and normal skin shows no statistical significance. It indicates that excessive proliferation and the lack of apoptosis of cells in dermis may cause the formation and development of hypertrophic scars and keloids.