葛根素抑制大鼠血管平滑肌细胞增殖及c-fos和bcl-2蛋白表达

目的：观察葛根素对血管平滑肌细胞（VSMC）增殖的影响及对c-fos和bcl-2蛋白的表达作用,旨在认识葛根素作用的分子机制。方法： 建立凝血酶（T）诱导VSMC增殖的模型,以细胞计数和流式细胞仪DNA含量测定、细胞周期分析法观察T及葛根素对VSMC增殖和DNA合成的影响。T及葛根素等各处理因素作用24 h后,用免疫印迹法（Western blot）检测c-fos蛋白和bcl-2蛋白表达。结果：T对VSMC有明显促增殖作用,促增殖效应在24 h 末达峰,且T在0.1～1.0 U·L^-1有剂量依赖关系；葛根素呈剂量依赖性地抑制T诱导的细胞增殖、DNA合成以及 VSMC c-fos和bcl-2蛋白的表达。结论： 葛根素能抑制T诱导的VSMC增殖,这可能与其抑制c-fos和bcl-2蛋白表达有关。

Puerarin suppresses the proliferation of vscular smooth muscule cells and c-fos and bcl-2 protein expression

OBJECTIVE: To observe the role of puerarin on the proliferation of vascular smooth muscle cells(VSMC) induced by thrombin (T) and the effect of puerarin on the c-fos and bcl-2 protein expression. METHOD: Cell number and cell cycle analysis using flow cytometry were adopted as two different indicators of effects on proliferation of VSMC. Western blot was used to indicate the changes of c-fos and bcl-2 protein after 24 h of treatment of T and puerarin. RESULT: 1.5 x 10(-5) - 1.5 x 10(-3) mol x L(-1) puerarin could significantly suppress this stimulation of VSMC proliferation and DNA synthesis induced by T. Western blot demonstrated that after 24 hour of treatment with T and puerarin, T could significantly increase c-fos and bcl-2 protein and 1.5 x 10(-5) - 1.5 x 10(-3) mol x L(-1) puerain could significantly suppress this increase. CONCLUSION: puerarin can suppress the proliferation and DNA synthesis of VSMC promoted by T. This inhibitory effects of puerarin are closely related with the suppression of c-fos and bcl-2 protein.