大鼠脑缺血再灌注后GDNF mRNA的表达及人参皂甙Rb1对其的影响

目的:从分子水平探讨大鼠局灶性脑缺血再灌注后的GDNFmRNA表达规律及人参皂甙Rb1对其的影响。方法:阻塞大鼠大脑中动脉2h再灌注3h至10d制备脑缺血模型,通过RT-PCR方法克隆的大鼠GDNFcDNA构建重组腺病毒质粒,并用质粒制备地高辛标记的GDNFcDNA探针,采用原位杂交技术观察脑缺血模型不同时程GDNFmRNA的表达及人参皂甙Rb1对其的影响。结果:GDNFmRNA主要分布于神经细胞的突起,在纤维束处更明显。脑缺血再灌注3h后GDNFmRNA表达出现上调反应,3d达高峰,此后逐渐减弱。人参皂甙Rb1能促进GDNFmRNA的表达。结论:脑缺血再灌注后GDNFmRNA的表达与缺血性损伤有一定的联系,人参皂甙Rb1对中枢神经系统有保护作用。

The expression of GDNF mRNA in rat after cerebral ischemia reperfusion and the effects of Ginsenoside Rb1 on it

Objective: To investigate the expression of GDNF mRNA in rat after cerebral ischemia reperfusion and the effects of Ginsenoside Rb1 on it on molecular level. Methods: The rat models of the cerebral ischemia were established by occluding middle cerebral artery for 2hours and reperfusing 3 hours ～10 days. The recombinant adenoviral plasmid was constructed by GDNF cDNA of rat which was cloned by RT-PCR, and the plasmid was used to produce GDNF cDNA probe labelled with digoxygenin. Adopting the technique of in situ hybridization, we observed the time course changes of GDNF mRNA on these models and the effects of Ginsenoside Rb1 on it. Results: GDNF mRNA was mainly located in neuronal processes, especially in fasciculus. GDNF mRNA upregulation was detected 3 hours after reperfusion, and reached the peak day 3, from then it decreased gradually. Ginsenoside Rb1 may improve the expression of GDNF mRNA. Conclusion: The expression of GDNF mRNA after cerebral ischemia reperfusion was related to ischemic injury, and Ginsenoside Rb1 has the protective effect to central nervous system.