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Enhancement of bovine mammary gland mononuclear cell Mitogenesis by recombinant bovine interleukin‐2 |
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| Authors: | P M Torre P K Konur S P Oliver |
| Institution: | 1. Department of Animal Science, Institute of Agriculture , University of Tennessee , Knoxville, TN, 37901–1071, USA;2. USDA/ARS Human Nutrition Research Center on Aging , Tufts University , 711 Washington St, Boston, MA, 02111, USA;3. Department of Animal Science, Institute of Agriculture , University of Tennessee , Knoxville, TN, 37901–1071, USA |
| Abstract: | Recombinant bovine interleukin‐2 was evaluated as a potential immunoenhancer of bovine mammary gland mononuclear cells. Bovine mononuclear cells were isolated from five primiparous Holstein cows at 14–18 and 28–32 days of involution and at 7–13 days prior to parturition. Optimal stimulatory concentrations of the T‐lymphocyte mitogen concanavalin A and the T‐dependent B‐lymphocyte mitogen pokeweed mitogen were determined to be 6.25 and 2 μg ml‐1 respectively. Proliferation of mononuclear cells in response to recombinant bovine interleukin‐2 was evaluated in the presence of suboptimal concentrations of concanavalin A and pokeweed mitogen to avoid masking potential effects of recombinant IL‐2. Bovine blood and mammary gland mononuclear cells were highly responsive to mitogens and to recombinant bovine interleukin‐2. The response of mammary gland mononuclear cells to recombinant bovine interleukin‐2 was comparable with the response of blood mononuclear cells. The data suggest that recombinant bovine interleukin‐2 may be an effective in vivo immunoenhancer of mammary gland mononuclear cells. |
| Keywords: | Mammary mononuclear cells proliferation recombinant bovine interleukin‐2 |