近红外光谱法快速检测维C银翘片中维生素C的含量

目的 应用近红外漫反射技术和化学计量学方法对维C银翘片中的维生素C进行快速定量分析.方法 采集样品的近红外漫反射光谱数据,采用偏最小二乘法(PLS)建立光谱数据和样品中维生素C含量的相关模型,应用所建模型对未知样品进行预测.结果 应用维生素C定量分析模型,以731个维C银翘片样品为校正集,浓度范围为86～ 201mg·...

Determination of ascorbic acid in the VC Yinqiao pius by near-infrared diffuse reflectance spectroscopy

OBJECTIVE A near-infrared quantitative method was developed for rapid determination of ascorbic acid in the VC Yinqiao pills.METHODS The near-infrared diffuse reflectance spectra of the samples was collected to establish a relative model between the spectra data and the ascorbic acid contents in VC Yinqiao pills by partial least squares regression,and then the samples were determined using the model.RESULTS For the quantitative model of ascorbic acid content,731 batches of VC Yinqiao pills were used as calibration set,the concentration range was 86～201mg·g-1,and 366 batches as validation set,the concentration range was 90～191mg·g-1.The root mean square error of cross validation(RMSECV) was 6.53 and R2=90.38,and root mean squar error of prediction(RMSEP) was 8.75 and R2=79.94.CONCLUSION The results show that the proposed method can be applied for fast detection of ascorbic acid in the VC Yinqiao pills.