尿激酶型纤溶酶原激活物途径在大鼠酒精性肝纤维化形成中的作用

目的 通过观察尿激酶型纤溶酶原激活物(uPA)及其抑制物(PAI-1)在大鼠酒精性肝纤维化形成中的动态变化,探讨uPA纤溶途径在酒精性肝纤维化形成中的作用.方法 雄性SD大鼠随机分为空白组、四氯化碳(CCl4)组和造模组.采用56度二锅头酒、玉米油、吡唑混合物灌胃联合腹腔注射CCl4橄榄油溶液(CCl4∶橄榄油=1∶3...

The role of urokinase-type plasminogen activator and type-1 plasminogen activator inhibitor fibrinolytic system in the development of alcoholic liver fibrosis in rats

Objective To study the role of urokinase-type plasminogen activator and type-1 plasminogen activator inhibitor fibrinolytic system in alcoholic liver fibrosis in rats by observing the dynamically variety of them in the development of alcoholic liver fibrosis.Methods SD rats were divided into three groups randomly：control group,CCl_4 group and experiment group.The experiment group was ingested the mixture（560 mL/L alcohol,10 mL/kg per day; corn oil 2 mL/kg per day;pyrazole 25 mg/kg per day） once a day and peritoneal injection 0.3 mL/kg 25%solution of CCl_4 in olive oil twice a week.The CC1_4 group received peritoneal injection as the experiment group and ingested saline （12 mL/kg per day）.Control group received peritoneal injection and ingested saline with the equal volume as the experiment group.The rats were anaesthetized and killed at the 4,8,10 and 12 weeks,The levels of ALT,AST in the serum were analyzed.Pathological changes in liver tissues were observed.The expression of uPA and PAI-1 were evaluated with Western blot,Immuno fluorescence,Real-time PCR.Results No significant difference was found between CCL group and control group.Compared with control group,the severity of liver fibrosis in the model group increased（P0.01）,and serum ALT,and AST levels increased as well,especially the AST（P0.05）;Also uPA and PAI-1 levels were significantly elevated,and PAI-1 increased more signficantly than uPA（P0.01）.Conclusion uPA fibrinolysis system plays a key role in the development of alcoholic liver fibrosis,As for the formation of alcoholic liver fibrosis,the amount of uPA and PAI-1 significantly increased,but PAI-1 increased much more obviously.