大鼠鞘内阿米洛利合用吗啡的抗骨癌镇痛作用

目的：探讨鞘内阿米洛利合用吗啡对大鼠骨癌的镇痛作用。方法：将walker256乳腺癌细胞注入SD雌性大鼠胫骨内形成骨癌痛模型，再行蛛网膜下腔置管。随机将大鼠分成对照组、阿米洛利组、吗啡组、混合药物组。鞘内给药前后测定大鼠机械性缩足反应阂值。采用线性回归的方法计算各药物的半数有效剂量（ED50）及95％的可信区间（c195）。使用Isobolographic评价药物的相互作用。结果：鞘内单独注射阿米洛利可产生剂量依赖性的抗骨癌痛作用，其半数有效剂量（ED。）及95％可信区间ck分别是：63．8μg和53．2—74．5μg。鞘内阿米洛利和吗啡合用可以产生协同镇痛作用。结论：鞘内单独注射阿米洛利、吗啡可以产生明显剂量依赖性的抗骨癌痛作用；鞘内注射阿米洛利可以增强吗啡的抗骨癌痛作用。

Effects of intrathecal amiloride alone or combined with mophine on bone cancer pain in a rat model

Objective：To investigate the analgesic effects of intrathecal amiloride alone or combined with morphine on bone cancer pain in rats. Methods ： Female SD rats were used for modeling of bone cancer pain by intratibial injection of walker-256 breast cancel cells. The rats received cannulation of subarachnoid space, and were then randomized into the control group, amiloride group, morphine group, and amiloride plus morphine group. Before and after intrathecal drug administration, paw withdrawal mechanical threshold （PWMT） were measured in the rats. Median effective dose （ EDs0 ） values and 95% confidence intervals （ CI95 ） were calculated using a least-square linear regression model. Isobolographic analysis was performed to evaluate the interactions between amiloride and morphine. Result：Intrathecal amiloride alone produced a dose-dependent analgesic effect against bone cancer pain, with EDs0 and CI95 being 63.8 wg and 53.2 - 74.5 vLg, respectively. Synergistic effects were found with intrathecal amiloride plus morphine. Conelusion：Intathecal amiloride or morphine alone may result in a dose-dependent effect against bone cancer pain. Synergistic effects may appear with combined intrathecal use of amiloride and morphine.