心肌梗死大鼠心脏ACE2基因的表达意义

目的:研究大鼠ACE2的mRNA在急性心肌梗死(AMI)后大鼠心脏组织中的表达. 方法:将36只SD雌性大鼠按时间段随机分为3组(每组12只),每组再随机分为假手术组、手术组2个亚组(每组6只),应用RT-PCR方法检测大鼠AMI后1,3,28 d左心室梗死区及非梗死区心肌ACE2 mRNA的表达,并以图像分析系统对其进行半定量分析. 结果:在AMI后1 d,与假手术组相比,手术组梗死区及非梗死区ACE2 mRNA水平均无差异(P》0.05). 在AMI后3 d,与手术组非梗死区相比,手术组梗死区ACE2 mRNA水平明显升高(P《0.01). 在AMI后28 d,与假手术组相比,手术组非梗死区ACE2 mRNA水平也明显升高(P《0.01). 结论:AMI后ACE2 mRNA表达增多.

Expression of ACE2 mRNA in rat myocardium after acute myocardial infarction and its significance

AIM: To investigate the changes of the mRNA expression of ACE2 after acute myocardial infarction(AMI) in SD female rat.METHODS: Thirty-six SD female rats were randomly separated into 3 groups(n=12 in each one) according to the infarction duration(1,3,28 d),which were randomly and evenly again separated into 2 subgroups: operated group and sham operated group.The levels of mRNA expression of ACE2 in the noninfarct and infract area of left ventricle 1,3,28 d after AMI were detected by RT-PCR and analyzed semi-quantitatively by image-analysis system. RESULTS: Compared with sham operated group,the mRNA expression of ACE2 had no significant difference in the infract and noninfarct areas of operated group at day 1 after AMI(P>0.05).The mRNA expression of ACE2 increased in the infract area compared with the noninfarct area at day 3 after AMI(P<0.01).At day 28,increase of ACE2 mRNA expression were also seen in the noninfarct area of AMI rats compared with the myocardium of control rats(P<0.01).CONCLUSION: The gene expression of ACE2 at mRNA level was significantly increased in the myocardium after AMI.