机械通气所致大鼠急性肺损伤对核内激素受体表达的影响

目的研究机械通气所致急性肺损伤对大鼠肺组织中核内激素受体（Nr1d1）表达的影响，探讨其在机械通气肺损伤中可能
的作用机制。方法72只大鼠分成4个时间点（1:00AM，7:00AM，1:00 PM，7:00 PM），每个时间点随机分成3组（FB组、LV组、HV
组），通气3 h 后，提取肺组织RNA，应用大鼠全基因组芯片和荧光定量实时PCR检测机械通气对Nr1d1 表达的影响。结果
基因芯片结果显示HV组Nr1d1表达下调，荧光定量实时PCR结果与芯片结果吻合；余弦分析Nr1d1基因在FB组具有节律性
（P<0.05），余未见明显节律性。结论Nr1d1表达随潮气量的增大而节律消失；Nr1d1表达下降与机械通气所致急性肺损伤密切
相关。

Pulmonary expression of Nr1d1 in rats with ventilation-induced lung injury

Objective To investigate the changes in the expression of nuclear receptor subfamily1 (Nr1d1) in the lungs of rats
with ventilation-induced lung injury and explore the molecular mechanism. Methods Seventy-two rats were randomly
divided into 4 groups for observation at 4 time points (1:00 AM, 7:00 AM, 1:00 PM and 7:00 PM), and each group was further
divided into 3 subgroups (Group FB, Group LV and Group HV). After ventilation for 3 h, the rats were sacrificed and lung
tissue samples were obtained for testing the expression of Nr1d1 using gene chip technology and RT-PCR. Results Nr1d1 gene
expression was down-regulated in HV group as shown by gene chip RT-PCR data. Cosine analysis showed that Nr1d1
expression exhibited a rhythmicity in FB group (P<0.05). Conclusion The rhythmicity of Nr1d1 expression disappears as the
tidal volume the increase. Diminished Nr1d1 expression is closely correlated with ventilation-induced lung injury in rats.