消瘕方抑制人乳腺癌裸鼠移植瘤血管内皮生长因子表达的实验研究

目的:研究消瘕方对人乳腺癌MDA-MB-435细胞株裸小鼠移植瘤中血管内皮生长因子(VEGF)表达的影响。方法:以体外培养的人乳腺癌MDA-MB-435细胞接种于裸小鼠右腋部位制造裸鼠皮下移植瘤模型,随机分为生理盐水组、5-氟尿嘧啶组及消瘕方小,中,大剂量组,每组小鼠予以相应药物治疗6周后脱颈处死,称瘤重,计算肿瘤生长抑制率,并采用RT-PCR法、免疫组化法检测肿瘤组织中VEGFmRNA及蛋白的表达。结果:消瘕方可抑制乳腺移植瘤生长,降低瘤体内VEGFmRNA表达(P<0.05),下调相应VEGF蛋白表达(P<0.05),其中以中剂量组效果更明显。结论:消瘕方的抑瘤作用与其下调瘤体内VEGF表达密切相关。

Effects of Xiaojia Decoction on Expression of Vascular Endothelial Growth Factor in Transplantation Tumors of Breast Cancer in Nude Mice

Objective: To study the effects of Xiaojia Decoction on expression of Vascular Endothelial Growth Factor in transplantation tumors of the breast cancer cell line MDA-MB-435 in nude mice.Methods: The MDA-MB435 human breast cancer cells cultured in vitro were subcutaneously inoculated into BALB/c nude mice to establish the transplanted tumor model.The mice were then randomized into five groups and treated with Xiaojia Decoction or other remedies for 6 weeks.Then we sacrificed the mice and weighed the tumor,calculated the inhibitory rate of tumor weight,the expression of VEGF on mRNA and protein level was determined by means of RT-PCR and immunohistochemical assay.Results: Xiaojia Decoction could inhibit the growth of tumor conspicuously,lower the expression of both mRNA and protein level of VEGF(P<0.05),especially for the medium-dose group.Conclusion: This Decoction's effects on down-regulating VEGF have close relation with its function of inhibiting the tumor growth.