HPLC-ELSD法测定白果中银杏内酯A、银杏内酯B和银杏内酯C

目的建立HPLC-ELSD法同时测定白果药材中银杏内酯A、银杏内酯B和银杏内酯C 3个萜杏内酯成分。方法采用Agilent 5TC C18色谱柱(250 mm×4.6 mm,5μm),以甲醇–四氢呋喃–水(25∶10∶65)为流动相,体积流量1 m L/min,柱温30℃,蒸发光散射检测器检测,对照品进样量为10、20μL,供试品溶液进样量为5~20μL。结果银杏内酯A在0.37~5.92μg、银杏内酯B在2.8~44.8μg、银杏内酯C在0.65~10.4μg时线性关系良好。银杏内酯A、银杏内酯B、银杏内酯C平均回收率分别为92.40%、95.03%、91.29%,RSD值分别为2.75%、2.06%、2.88%。结论本法操作简单,重复性好,为白果药材的质量控制提供了依据。

Determination of ginkgolide A, ginkgolide B, and ginkgolide C in Ginkgo Semen by HPLC-ELSD

Objective To establish an HPLC-ELSD method for the determination of three terpene lactones ginkgolide A, ginkgolide B, and ginkgolide C in Ginkgo Semen. Methods The determination was carried out on Agilent 5TC C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol–tetrahydrofuran-water (25∶10∶65). The column temperature was set at 30 ℃ at a flow rate of 1.0 mL/min. ELSD was used as detector to determine the terpene lactones. The injection volumes were 10 and 20μL for reference substance solution, and 5 — 20μL for sample solution. Results The linear ranges of ginkgolide A, ginkgolide B, and ginkgolide C were 0.37 — 5.92μg, 2.8 — 44.8μg, and 0.65 — 10.4μg, respectively. The average recoveries of three components were 92.40%, 95.03%, and 91.29% with RSD 2.75%, 2.06%, and 2.88%, respectively. Conclusion The method is simple and repeatable, which can be used in quantity control for Ginkgo Semen.