泌尿生殖道感染者性传播疾病病原体荧光定量PCR检测分析

目的了解淋球菌(NGH)、沙眼衣原体(CT)、解脲脲原体(Uu)、单纯疱疹病毒(HSV-Ⅱ)、人乳头瘤病毒(HPV6,11)5种性传播疾病(STD)病原体的检出情况,为临床预防提供依据。方法采用实时荧光定量PCR法对该院门诊2 029例患者进行NGH、CT、Uu、HSV-Ⅱ、HPV6,11病原体DNA定量检测。结果 STD病原体总阳性率为24.15%(490/2 029),HPV6,11、Uu、HSVⅡ、CT和NGH阳性率分别为39.53%(34/86)、36.85%(276/749)、28.49%(51/179)、17.92%(93/519)和7.26%(36/496);男和女阳性率分别为20.34%和33.92%;21～40岁年龄段患者占85.51%。结论加强泌尿生殖道炎症患者STD病原体的检测,对STD的防治有重要意义。

Analysis on pathogens of sexually transmitted diseases of patients with genitourinary tract infection by fluorescence quantitative PCR detection

Objective To learn about the infection status of 5 sexually transmitted diseases（STD） pathogens of NGH,CT,Uu,HSV-Ⅱ,HPV6,11 and provide evidence for the prevention and treatment of these STDs.Methods 2 029 samples of outpatients were detected the NGH,CT,Uu,HSV-II and HPV6,11 by fluorescence quantitative PCR detection（FQ-PCR）.Results Total positive rate of STD pathogens was 24.15%,the positive rates of HPV6,11,Uu,HSV-Ⅱ,CT and NGH were 39.53%（34/86）,36.85%（276/749）,28.49%（51/179）,17.92%（93/519）and 7.26%（36/496）.The positive rates of male and female samples were 20.34% and 33.92% respectively.The patients aged 21 to 40 years accounted for 85.51%.Conclusion Enhancing detection of STD pathogens is important for its prevention and treatment.