肿瘤转移抑制基因KAI1/CD82在不同转移潜能癌细胞中的表达

目的 探讨新发现的肿瘤转移抑制基因ＫＡＩ１／ＣＤ８２与人癌细胞转移潜能的关系，方法 应用Ｎｏｒｔｈｅｒｎｂｌｏｔ杂交及聚合酶链反应－单链构象多态性分析（ＰＣＲ－ＳＳＣＰ）方法，检测３组８种不同转移具有转移性的前列腺癌细胞系ＰＣ３和ＰＣ３Ｍ及高转移性的人肺ＰＧ细胞，ＫＡＩ１ｍＲＮＡ的表达降低（积分吸光度分别为０．０３１９，０．０２６６和０．０５４９）而在无转移性的人肺ＰＡａ细胞则为高表达（积分吸光度

Expression of tumor metastasis suppressor gene KAI1/CD82 in human cancer cell lines with different metastasis potential

Objective To investigate the correlation of a newly defined tumor metastasis suppressor gene KAI1/CD82 and metastatic potential of human cancer cell lines Methods Expressions of KAI1/CD82 in eight human cancer cell lines of prostate, lung and melanoma with different metastasis potential were studied by Northern blot hybridization and the mutation were detected by PCR SSCP Results Expressions of KAI1 mRNA decreased in human prostate cancer cell lines PC3 and PC3M, and lung cancer cell line PG which were metastatic cancer cell lines (integral light density values: 0 031 9, 0 026 6 and 0 054 9, respectively) Expression of KAI1 mRNA showed high level in PAa, a human lung cancer cell line with no metastasis (integral light density value: 0 731 3) In human melanoma cell lines with different metastasis potential (WM35,WM1341b,WM983a and WM451), expression of KAI1 showed moderate expression (integral light density values are 0 179 8, 0 158 2, 0 150 1, 0 180 0 respectively) PCR SSCP analysis indicated the existence of band shift in exon 7 of KAI1 gene in PC3,PG and PC3M Conclusions Decreased KAI1/CD82 expression may be associated with enhanced metastatic potential in prostate cancer cell lines and lung cancer cell line other than melanoma cell lines The decreased expression may be partially nelaled with mutation of KAI1 gene