| 新维甲类化合物5-4A抗癌细胞侵袭作用及其机理研究 |
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| 引用本文： | 曹西华 韩锐. 新维甲类化合物5-4A抗癌细胞侵袭作用及其机理研究[J]. 中华医学杂志, 1999, 79(8): 626-627 |
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| 作者姓名： | 曹西华 韩锐 |
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| 作者单位： | 中国医学科学院中国协和医科大学药物研究所 |
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| 摘 要： | 目的 为探讨新维甲类化合物５４ Ａ 对癌细胞侵袭能力的影响及其作用机理。方法利用体外重组基底膜侵袭试验，观察癌细胞的侵袭能力；应用生物化学及形态学方法，检查细胞的恶性表型。结果 ５４ Ａ 在４ ×１０ － ５ ｍｏｌ／ Ｌ浓度时，对黑色素瘤高转移株 Ｂ１６ Ｂ Ｌ６ 细胞膜侵袭具有显著的抑制作用，抑制率为３３９ ％ 。结论 ５４ Ａ 具有抗 Ｂ１６ Ｂ Ｌ６ 膜侵袭作用。其作用与其抑制肿瘤细胞的运动能力、Ⅳ型胶原酶活力以及改变肿瘤细胞的恶性表型有关。
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| 关 键 词： | 维甲类 药物筛选试验 5－4A 抗癌作用 |
Inhibition of tumor invasion by new retinoid, 5 4A, and its mechanism of the action |
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| CAO Xihua,HAN Rui Institute of Material Medical,Chinese Academy of Medical Sciences,Peking Union Medical University,Beijing. Inhibition of tumor invasion by new retinoid, 5 4A, and its mechanism of the action[J]. Zhonghua yi xue za zhi, 1999, 79(8): 626-627 |
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| Authors: | CAO Xihua HAN Rui Institute of Material Medical Chinese Academy of Medical Sciences Peking Union Medical University Beijing |
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| Affiliation: | Institute of Material Medical, Chinese Academy of Medical Sciences, Peking Union Medical University, Beijing 100050. |
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| Abstract: | OBJECTIVE: To investigate the effect of 5-4A on the invasion of B16BL6 and to explore its mechanisms of action. METHODS: The invasion of B16BL6 was studied using the reconstituted basement membrane invasion assay. RESULTS: The phenotype of B16BL6 cells was obviously suppressed by 5-4A at the concentration of 4 x 10(-5) mol/L, and the inhibitory rate was 33.9%. CONCLUSION: 5-4A can inhibit invasion of B16BL6 cells and its mechanisms of action might be associated with the suppression of movement of tumor cells, decrease in the expression of type IV collagenase, and reversion of the phenotype of B16BL6 cells. |
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| Keywords: | Retinoid Drug screening assays antitumor |
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