霍乱弧菌及其他致病弧菌DNA多态性的研究

目的 探讨霍乱弧菌和其它致病弧菌分子遗传特征及相互关系。方法 用聚合酶链反应（ＰＣＲ），ＤＮＡ序列分析，随机扩增多态性ＤＮＡ（ＲＡＰＤ）和平行链锁聚类分析，对３株Ｏ１群古典型，４株Ｅ１ Ｔｏｒ型，３株Ｏ１３９群霍乱弧菌和副溶血等３株致病菌进行检测。结果 Ｏ１３９群霍乱弧菌和Ｏ１群含有的霍乱肠毒素（ＣＴＸ）基因，核苷酸序列同源性为９６．９％－９８．７％。

DNA polymorphism of Vibrio Cholera and other Vibrios

OBJECTIVE: To investigate the inherent characteristics of V. cholera and other vibrios and the association between them. METHODS: Polymerase chain reaction (PCR), DNA sequences analysis, randomly amplified polymorphic DNA (RAPD) and average linkage cluster analysis were used to study V. cholera including 3 strains of O139, 4 strains of E1 Tor biotype, 3 strains of classical and 3 other vibrios. RESULTS: V. cholera O139 contained the genomic sequences of CTX A2-B as well as O1 V. cholera, indicating that their homologous nucleotides was 96.9%-98.7%, and other vibrios did not contain CTX. O1 V. cholera and other vibrios were divided into 4 groups by RAPD: classical; O139 and E1 Tor; V. paraheamolyticus and V. vulnificus; and V. flluvialis. The O139 DNA fingerprints of RAPD were almost as same as E1 Tor biotype, whose average linkage cluster distance was 0, similar with classical biotype, whose average linkage cluster distance was 2.07, much different from V. paraheamolyticus and others with the average linkage cluster distance being 6.76-8.54. CONCLUSION: V. cholera and other vibrios are polymorphic in inherent characteristics. Being as same as the inherent characteristics of E1 Tor, O139 may evoluted from E1 Tor biotype. And also the inherent characteristics of V. parahemolytisus were as same as those of V. vulnificus.