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幽门螺杆菌空泡毒素基因的单链构象多态性研究
引用本文:Peng H,Pan G,Chao S. 幽门螺杆菌空泡毒素基因的单链构象多态性研究[J]. 中华医学杂志, 1999, 0(3): 181-184
作者姓名:Peng H  Pan G  Chao S
作者单位:中国医学科学院中国协和医科大学北京协和医院消化科,中国医学科学院中国协和医科大学基础医学研究所分子生物学国家重点实验室
摘    要:目的 应用PCR/SSCP分析幽门螺杆菌(Hp)的空泡毒素基因(vacA)多态性,借此区分不来源的Hp。方法 用vacA为模板设计的引物对159例各种胃,十二指肠疾病患者胃粘膜行PCR扩增,其产物作SSCP分析和Southern印迹杂交,对3例具不同SSCP带型的十二指肠溃疡(DU)患者的Hp进行基因序列分析。结果 vacA的两种产物vacA1和vacA2各占76.5%(114/149)和23.5

关 键 词:幽门螺杆菌 空泡毒素基因 单链构象 胃肠疾病

A single strand comformation polymorphism of vacuolating cytotoxin gene in H. pylori
Peng H,Pan G,Chao S. A single strand comformation polymorphism of vacuolating cytotoxin gene in H. pylori[J]. Zhonghua yi xue za zhi, 1999, 0(3): 181-184
Authors:Peng H  Pan G  Chao S
Affiliation:Department of Gastroenterology, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Beijing, 100730.
Abstract:Objective To use PCR/SSCP analysis of the vacuolating cytotoxin gene (vacA) of H.pylori for differentiation of various strains of H. pylori.Methods PCR was performed using the primers amplifing vacA gene of the bacteria embeded in the gastric mucosa of 159 patients with various gastric duodenal diseases. The products of PCR were futher processed for SSCP analysis and southern blot hybridization. In the meantime, vacA genes of three different SSCP patterns from three patients with duodenal ulcers were sequenced. Results The rate of detection of H.pylori with the method was 100%. vacA 1 and vacA 2, the two subtypes of vacA, were 76.5%(114/149) and 23.5%(35/149), respectively. Eight different SSCP patterns were distributed in various gastroduodenal diseases, and that 80% of duodenal ulcers was predominated with B pattern. Sequencing of DNA indicated a diversity of vacA gene structure. Conclusion PCR/SSCP can be used in the differentation of different strains of H. pylori in epidemology, and in the follow up study after H.pylori eradication, especially in the differentiation between H. pylori recrudescence and reinfection.
Keywords:Helicobactor pylori Cytotoxicity   immunologic Gene Single strand comformation polymorphism  
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