| HPLC法测定扶桑花中槲皮素的含量 |
| |
| 引用本文： | 钟宇森,张春荣. HPLC法测定扶桑花中槲皮素的含量[J]. 广东药学院学报, 2010, 26(1): 45-47. DOI: 10.3969/j.issn.1006-8783.2010.01.013 |
| |
| 作者姓名： | 钟宇森 张春荣 |
| |
| 作者单位： | 广东药学院,中药学院,广东,广州,510006;广东药学院,中药学院,广东,广州,510006 |
| |
| 基金项目： | 广东药学院科研启动基金 |
| |
| 摘 要： | 目的建立扶桑花中槲皮素的含量测定方法。方法采用HPLC法,色谱柱为Dikma platisil ODS柱（250 mm×4.6 mm,5μm）,流动相为甲醇-0.5%磷酸水溶液（体积比30∶70）,流速1.0 mL·min^-1,柱温30℃,检测波长360 nm。结果槲皮素进样量在0.041 6-0.416μg范围内与峰面积呈良好的线性关系,平均回收率为98.95%,RSD为1.09%（n=6）。结论该方法操作简便、快速、准确,可用于扶桑花药材的质量控制。
|
| 关 键 词： | 扶桑花 槲皮素 高效液相色谱法 |
Determination of quercetin in Flos Hibisci Rosae-sinensis by HPLC |
| |
| ZHONG Yu-sen,ZHANG Chun-rong. Determination of quercetin in Flos Hibisci Rosae-sinensis by HPLC[J]. Academic Journal of Guangdong College of Pharmacy, 2010, 26(1): 45-47. DOI: 10.3969/j.issn.1006-8783.2010.01.013 |
| |
| Authors: | ZHONG Yu-sen ZHANG Chun-rong |
| |
| Affiliation: | ZHONG Yu-sen,ZHANG Chun-rong (School of Traditional Chinese Medicine,Guangdong Pharmaceutical College,Guangzhou,Guangdong 510006,China) |
| |
| Abstract: | Objective To develop a method for the determination of quercetin in Flos Hibisci Rosae-sinensis.Methods HPLC was performed using a Dikma platisil ODS column（250 mm×4.6 mm,5 μm） with methanol-0.5% phosphoric acid solution（30∶70） as mobile phase under a flow rate of 1.0 mL·min^-1.The column temperature was 30 ℃,and the detection wavelength was 360 nm.Results The calibration curve of quercein was linear within the range of 0.041 6-0.416 μg,and the average recovery was 98.95% with the RSD of 1.09%（n=6）.Conclusion This method is convenient,reproducible and accurate,and can be used in quality control of Flos H.Rosae-sinensis. |
| |
| Keywords: | Flos Hibisci Rosae-sinensis quercetin HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
