Effects of deltamethrin on the antioxidant defense and heat shock protein expression in Chinese mitten crab,Eriocheir sinensis |
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| Affiliation: | 1. State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, PR China;2. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, PR China;3. Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture and Rural Affairs, Beijing, 100000, PR China;4. Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Environments Factors (Wuxi), Ministry of Agriculture and Rural Affairs, Wuxi, 214081, PR China;1. Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences & College of Marine Science and Engineering, Nanjing Normal University, 1 Wenyuan Road, Nanjing, 210023, China;2. Wujin Aquatic Technology Promotion Station, Changzhou, 213017, Jiangsu, China;3. Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, Jiangsu, China |
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| Abstract: | In the present study, the status of antioxidant response and molecular regulation in Chinese mitten crab, Eriocheir sinensis under the exposure of synthetic pyrethroid deltamethrin were investigated by means of measuring the antioxidative enzyme activity and relative mRNA expression of heat shock proteins (HSPs) in hepatopancreas. The results showed that activity of superoxide dismutase (SOD) and catalase (CAT) decreased remarkably in all treatments except the SOD activity at concentration of 0.073 μg/L. The oxidative stress products malondialdehyde (MDA) and hydrogen peroxide (H2O2) increased significantly at high concentrations while no significant difference was observed at concentrations of 0.073 and 0.146 μg/L throughout the experiment. Meanwhile, the relative mRNA expression of HSP 60, HSP 70 and HSP 90 was significantly up-regulated in all treatments at each time point. All resutls above indicated that deltamethrin has prominent toxic effect on E. sinensis based on antioxidative enzyme inhibition and oxidative products accumulation at environmental related concentrations, and a protective response by up-regulation of HSPs was carried out by animals to mitigate the oxidative stress. In addition, SOD, CAT, MDA, H2O2 and the expression of heat shock proteins, especially HSP 70 in hepatopancreas could be sensitive biomarkers in the assessment of toxic effect of deltamethrin on E.sinensis. |
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| Keywords: | Deltamethrin Oxidative stress Heat shock protein |
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