大承气汤对内毒素血症小鼠血浆内皮素-1及肾功能的影响

目的观察中药大承气汤对内毒素血症血浆内皮素-1及肾功能的影响。方法66只小鼠均分为三组：对照组，内毒素血症组（腹腔注射内毒素，立即、4h用生理盐水灌胃），和治疗组（腹腔注射内毒素，立即、4h用大承气汤灌胃）。注射4h、8h后眼眶取血测定血清血浆内皮素-1（ET-1）和血清肌酐。结果注射内毒素后4和6h，治疗组血浆内皮素一1水平分别为（363．669±31．747）pg／ml和（323．320±43．327）pg／ml，均显著低于内毒素血症组[（470．028±49．430）和（462．917±20．13）pg／ml，均P〈0．01]。注射内毒素后4和8h，治疗组血清肌酐水平分别为（40．1±3．71）μmol／L和（39．29±3．71）μmol／L，均显著低于内毒素血症组[（48．18±5．15）和（47．38±4．14）μmol／L，均P〈0．01]。结论大承气汤能降低内毒素血症血浆内皮素水平和血清肌酐水平，减轻肾脏损伤，其机制可能与抑制内皮素有关。

Effects of Dachengqi Decoction on plasma endothelin-1 and serum creatinin in endotoxemia: experiment with mice

Objective To investigate the effects of Dachengqi Decoction （DD） on plasma endothelin-1 and renal function in endotoxemia. Methods Sixty-six mice were randomly divided into 3 equal groups： lipopolysaccharide （LPS） group [undergoing intraperitoneal injection of LPS and gastic perfusion of normal saline （NS） immediate and 4 h after], DD treatment group （undergoing intraperitoneal injection of LPS and gastic perfusion of DD immediate and 4 h after）, and control group （undergoing intraperitoneal injection of NS）. Blood samples were collected from the eye sockets 4 and 8 hours after the introperitoneal injection to test the plasma endothelin-1 （ET-1） and serum creatinin. Results The plasma ET-1 levels 4 and 8 hours later of the DD group were （363.669±31.747） and （323.320＋±43.327） pg/ml respectively, both significantl7 lower than those of the endotoxemia group [（470.028±49.430）and（462.917±20.13） pg/ml respectively, both P〈0.01 ]. The serum creatinin levels 4 and 8 hours later of the DD group were （40.1±3.71） and （39.29±3.71） μmol/L, both significantly lower than those of the endotoxemia group [ （48.18±5.15） and（47.38±4.14） μmol/L respectively, both P〈0.01]. Conclusion DD decreases the levels of plasma ET-1 and serum creatinin, thus reducing the renal injury with the probable mechanism of inhibiting ET- 1 level.