| 矢车菊素-3-葡萄糖苷抑制体内外胰腺癌生长的实验观察 |
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| 引用本文： | 王海波,蔡慎初,徐晓峰,吴式琇,黄庆科. 矢车菊素-3-葡萄糖苷抑制体内外胰腺癌生长的实验观察[J]. 医学研究杂志, 2011, 40(10): 65-69 |
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| 作者姓名： | 王海波 蔡慎初 徐晓峰 吴式琇 黄庆科 |
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| 作者单位： | 325000,浙江省温州医学院附属第一医院 |
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| 基金项目： | 浙江省中医药管理局基金 |
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| 摘 要： | 目的探讨矢车菊素-3-葡萄糖苷(C3G)抑制体内外胰腺癌生长的作用及其机制,为合理利用C3G治疗胰腺癌提供理论基础。方法对人胰腺癌细胞株BxPC-3进行体外培养,应用不同浓度(2.5、5和10μg/ml)的C3G处理胰腺癌细胞后。光学显微镜下观察细胞形态变化;Cell counting kit-8(CCK-8)法检测细胞增殖;流式细胞仪检测细胞的凋亡率;Westernblotting检测胰腺癌细胞中XIAP和Smac蛋白表达;建立起裸鼠胰腺癌皮下移植瘤模型,观察C3G对裸鼠胰腺癌皮下移植瘤生长的影响;免疫组织化学法检测肿瘤组织中XIAP和Smac的阳性表达。结果与对照组相比较,C3G可呈浓度依赖性抑制体外胰腺癌BxPC-3细胞生长,并显著诱导细胞凋亡;C3G可分别上调和下调BxPC-3细胞中XIAP和Smac的表达;C3G可显著抑制裸鼠胰腺癌皮下移植瘤生长;C3G可分别明显降低和促进胰腺肿瘤组织中XIAP和Smac的阳性表达。结论 C3G可显著抑制体内外胰腺癌生长,该作用可能通过抑制XIAP和促进Smac蛋白表达而实现。
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| 关 键 词： | 胰腺癌 矢车菊素-3-葡萄糖 凋亡 |
| 收稿时间： | 2011-02-27 |
| 修稿时间： | 2011-03-03 |
Effect of Cyanidin-3-glucoside on Pancreatic Cancer |
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| Wang Haibo,Cai shenchu,Xu Xiaofeng,Wu Shixiu,Huang Qingke. Effect of Cyanidin-3-glucoside on Pancreatic Cancer[J]. Journal of Medical Research, 2011, 40(10): 65-69 |
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| Authors: | Wang Haibo Cai shenchu Xu Xiaofeng Wu Shixiu Huang Qingke |
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| Affiliation: | Wang Haibo,Cai shenchu,Xu Xiaofeng,Wu Shixiu,Huang Qingke.The First Affiliated Hospital of Wenzhou Medical College,Zhejiang 325000,China |
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| Abstract: | Objective To explore the effect and the mechanism of cyanidin-3-glucoside(C3G) in the growth inhibition of pancreatic cancer so as to provide basic rationales for its application in pancreatic cancer treatment.Methods After human pancreatic cancer cell line BxPC-3 was treated with different concentrations of C3G,cells morphological changes were observed under the optical microscope.The cellular proliferation was detected by cell counting kit-8(CCK-8) assay.The flow cytometry(FCM) was used to determine apopt... |
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| Keywords: | Pancreatic neoplasm Cyanidin-3-glucoside Apoptosis |
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