Increased expression and altered intracellular distribution of adhesion/growth-regulatory lectins galectins-1 and -7 during tumour progression in hypopharyngeal and laryngeal squamous cell carcinomas |
| |
Authors: | Saussez S Decaestecker C Lorfevre F Chevalier D Mortuaire G Kaltner H André S Toubeau G Gabius H-J Leroy X |
| |
Institution: | Laboratories of Anatomy;and Histology, Faculty of Medicine and Pharmacy, University of Mons-Hainaut, Mons, Belgium;, Laboratory of Toxicology, Institute of Pharmacy, Free University of Brussels (ULB), Brussels, Belgium, Departments of;Oto-Rhino-Laryngology;and Pathology, Faculty of Medicine, Hôpital Claude Huriez and Centre de Biologie-Pathologie –CHRU, Lille, France;, and Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, Munich, Germany |
| |
Abstract: | Aims: To examine the level of expression of the pleiotropic regulators galectins-1 and -7 in relation to neoplastic progression of hypopharyngeal (HSCCs) and laryngeal (LSCC s ) squamous cell carcinomas. Methods and results: The presence of galectins-1 and -7 was investigated using quantitative immunohistochemistry in (i) a series of 78 HSCCs by comparison with 17 normal epithelia (N_E), 26 low-grade dysplasia (low_D) and 27 high-grade dysplasia (high_D) and (ii) a series of 56 LSCCs by comparison with 50 N_E, 23 low_D and 29 high_D. Galectin-1 positivity expressed as a percentage of cells was significantly higher in carcinomas (HSCCs and LSCCs) than in N_E, low _ D or high _ D ( P < 10−6). Galectin-7 expression was elevated in low _ D ( P = 0.0004) compared with N_E and in carcinomas (HSCC) compared with high _ D ( P = 0.0002). Tumour progression from high_D to carcinomas was associated with a shift of galectin-1 localization from the nucleus towards the cytoplasm. Increased expression of galectin-7 in dysplasias was accompanied by a shift from the cytoplasmic compartment (N_E) to the nucleus (low_D and high_D). Conclusions: Our data reveal an association between the level of presence of galectins-1 and -7 and neoplastic progression of HSCCs and LSCCs. Moreover, inverse shifts between nuclear and cytoplasmic positivity intimating functional divergence were detected. |
| |
Keywords: | dysplasia epithelium lectin malignancy preneoplasia |
本文献已被 PubMed 等数据库收录! |
|