神经节苷脂对缺氧缺血新生鼠脑保护作用的研究

目的探讨神经节苷脂GM-1对缺氧缺血新生鼠脑组织超氧化物岐化酶、丙二醛以及神经元凋亡的影响。方法7日龄Wistar大鼠70只，随机分成3组，①假手术组：仅做颈正中切口，不做颈总动脉结扎；（函治疗组（GM一1组），缺氧缺血性脑损伤（HIBD）后即刻腹腔内注射GM-110mg／kg；③对照组（生理盐水组），HIBD后即刻腹腔注射等体积的生理盐水。①于手术后即刻，②、③于处置后0、24、48、72h断头取脑，分别检测SOD／MDA的水平，并经HE和Tunel染色，光镜下检测海马CAI脑细胞凋亡数。结果观察组24、48、72h脑组织SOD的水平明显高于对照组，MDA的水平明显低于对照组，两组差异有显著性（P〈0．01），对照组大鼠海马CA1区可见典型的凋亡细胞，表现为细胞固缩、胞浆深染、核浓缩、碎片、核浆分布不清，有凋亡小体形成；GM-1组上述凋亡细胞明显减少；Tunel染色对照组阳性细胞数与GM-1组比较差异有明显的统计学意义（P〈0．05）。结论GM-1对HIBD有明显的保护作用。

The study on protective effect of Gangliosides GMI on rat with hypoxic-ischemia

Objective : To explore the influence of the Gangliosides GM-1 on the hypoxic-ischemic neonatal rat model.Methods:70 postnatal 7-day-old Wistar rats were randomly divided as follows:group 1(n=10),sham operation;group 2(n=30),GM-1 group,and gsoup 3(n=30),normal saline.GM-1(10 mg/kg)or normal saline was injected intraperitoneally into rats in group 2 or 3 after hypoxia(left carotid artery ligation)and ischemia.The rats were killed in 0,24,48 and 72 hours after hypoxic-ischemia to detect the level of SOD and NDA in rat brain and neuronal apoptosis in the hippocampal CAI regin was detected by HE and TUNEL staining.Results:In group 2,the level of SOD was higher than that in group 3;but the level of MDA was lower than that in group 3.In group 1,few apoptotic cells were detected.Average TUNEL-positive cells in group 2 were much more than more than those in group 3,there was significant difference between the two groups(P<0.01).Conclusion: GM-1 can protect the brain of rats with HIBD.