| 开心散抑制过氧化氢诱导的PC12细胞凋亡的机制 |
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| 引用本文： | 钱云飞,姚文兵,王华,高向东.开心散抑制过氧化氢诱导的PC12细胞凋亡的机制[J].中国天然药物,2007,5(5):379-384. |
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| 作者姓名： | 钱云飞 姚文兵 王华 高向东 |
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| 作者单位： | 中国药科大学生命科学与技术学院,南京,210009 |
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| 基金项目： | 教育部高等学校优秀青年教师教学科研奖励计划;国家中医药管理局中医药科学技术研究基金 |
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| 摘 要： | 目的:研究开心散(Kaixin San,KXS)对过氧化氢(H2O2)诱导的PC12细胞凋亡的影响,并探讨其抗凋亡的机制。方法:以过氧化氢诱导的PC12细胞凋亡为模型,采用MTT比色法,琼脂糖凝胶电泳分析,荧光染色观察及流式细胞技术,考察KXS对凋亡的保护作用;采用比色法测定caspase活性、Western blot技术检测胞浆中细胞色素C水平,RT-PCR技术分析Bcl-2和Bax mRNA的表达,考察KXS抗凋亡的机制。结果:KXS可以有效地改善H2O2诱导的PC12细胞的凋亡,抑制caspase-3、caspase-9的活性,降低胞浆中细胞色素C的水平,增加Bcl-2mRNA的表达,减少BaxmRNA的表达,从而导致Bcl-2/Bax比值的增加。结论:KXS可能通过线粒体途径抑制H2O2诱导的PC12细胞的凋亡。
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| 关 键 词： | 开心散 PC12细胞 凋亡 阿尔茨海默病 |
| 文章编号： | 1672-3651（2007）05-0379-06 |
| 修稿时间： | 2007-03-05 |
The Mechanism of Kaixin San Inhibiting Apoptosis in Hydrogen Peroxide-induced PC12 Cells |
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| QIAN Yun-Fei,YAO Wen-Bing,WANG Hua,GAO Xiang-Dong.The Mechanism of Kaixin San Inhibiting Apoptosis in Hydrogen Peroxide-induced PC12 Cells[J].Chinese JOurnal of Natural Medicines,2007,5(5):379-384. |
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| Authors: | QIAN Yun-Fei YAO Wen-Bing WANG Hua GAO Xiang-Dong |
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| Institution: | School of Life Science and Technology,China Pharmaceutical University,Nanjing 210009,China |
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| Abstract: | AIM:To investigate the effect of Kaixin San (KXS) in H2O2-induced PC12 cells apoptosis and its mechanism of anti-apoptosis. METHODS: H2O2-induced PC12 cells apoptosis model was established, and the protective effect of KXS was assayed by MTT, Hoechst 33,258 staining, agarose gel electrophoresis and flow cytometric. Furthermore, the anti-apoptosis mechanism of KXS was investigated using Caspase assay, western blot and RT-PCR. RESULTS: KXS could protect the PC12 cells from the damage of H2O2, inhibit apoptosis by suppression of cytochrome C release and caspase cascade activation, as well as regulation of Bcl-2 family members. CONCLUSION: KXS may inhibit apoptosis in H2O2-induced PC12 cells from the mitochondria-mediated pathway. |
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| Keywords: | H2O2 |
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