宫颈上皮内瘤变石蜡标本 hTERC 基因检测与高危型人乳头瘤病毒分型测定的临床意义

目的：探讨宫颈上皮内瘤变石蜡标本中人染色体端粒酶基因（ hTERC）表达情况及其与高危型人乳头瘤病毒测定的临床意义。方法收集2009年10月至2012年10月湖北民族学院附属民大医院及暨南大学附属第一医院148例宫颈石蜡组织标本，其中对照组20例（正常宫颈），研究组128例（ CINⅠ级30例，CINⅡ级53例，CINⅢ级45例）。应用荧光原位杂交（ FISH）技术检测其hTERC基因的表达并采用凯普HPV－DNA分型测定上述受试者高危型人乳头瘤病毒感染情况。结果（1）随着宫颈病变级别增高，hTERC基因阳性表达率增加。不同级别宫颈上皮内瘤变hTERC基因扩增率与正常对照组比较，差异有显著统计学意义（χ^2＝63．707，P ＜0．05）； CINⅡ级及以上病变的hTERC基因扩增率明显高于CINⅠ级，差异有显著统计学意义（χ^2＝36．973，P＜0．05）。（2）受试者中高危型HPV感染阳性率为74．3％（110／148），高危型HPV感染阳性组hTERC基因扩增率和阴性组相比较，差异具有统计学意义（χ^2＝26．9，P＜0．05）。结论宫颈上皮内瘤变组织中hTERC基因扩增与HPV感染具有一定相关性。

An A nalysis on Detection of hTERC Gene in Paraffin Embedded Specimens and Human Papillomavirus Typing Detection for Cervical Intraepithelial Neoplasia Screening

Objective To explore genomic amplification of the human telomerase gene （ hTERC） in cervical intraepithelial neoplasia （ CIN） ,and demonstrate the clinical significance with the high-risk human papilloma virus detection.Methods From October 2009 to October 2012,148 cases of cervical tissue paraffin specimens were collected from first affiliated hospital of Jinan Univer-sity,including 20 cases of normal cervix in control group,128 cases in test group（ CINⅠ30 ca-ses, CINⅡ53 cases,CIN Ⅲ45 cases ） .Of which the hTERC gene expression were detected by fluorescence in situ hybridization （ FISH） and the high-risk HPV infection were detected by using flow-through hybridization an d gene chip technology.Results （ 1） With the increase of the grade of cervical lesions, the positive expression rate of hTERC gene increased.Compared with control group,the hTERC gene′s amplification rates of cervical intraepithelial neoplasia（ CIN）Ⅰ, CINⅡand CINⅢwere statistically significant （χ^2=63.707,P〈0.05） .The hTERC gene′s amplifi-cation rates of CINⅡ and CINⅢ were significantly higher than those of CINⅠ and the differ-ence was significant（χ^2=36.973,P〈0.05.（ 2） The positive rate of HPV infection in subjects was 74.3%（ 110/148）;Comparing the hTERC gene′s amplification rates of the high-risk HPV infec-tion positive group with that of the negative group,the difference was statistically significant（χ^2=26.9,P〈0.05） .Conclusion The hTERC gene amplification is associated with HPV infection.