| 地高辛标记PCR-SSOP法进行HLA-A02等位基因检测 |
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| 引用本文： | 翟宁,韩秀萍,贺卫东.地高辛标记PCR-SSOP法进行HLA-A02等位基因检测[J].中国医科大学学报,2000,29(3):215-216,219. |
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| 作者姓名： | 翟宁 韩秀萍 贺卫东 |
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| 作者单位： | 中国医科大学第一临床学院皮肤科!沈阳110001(翟宁，贺卫东，宋芳吉)，第二临床学院皮肤科(韩秀萍) |
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| 摘 要： | 目的：建立聚合酶链反应－序列特异笥革酸探讨（ＰＣＲ－ＳＳＯＰ）分型方法，并分析其「实用价值。方法：采用一对引物扩增所有ＨＬＡ－Ａ２特生抗原法和黄嘌呤用２３个探针和扩增产物杂文，探针用地高辛系统标记和检测，可鉴定Ａ２的２５个等位基因，并在中国人群ＨＬＡ－Ａ＾＊０２等位基因的检测中进行应用，用Ｂ淋巴母细胞朱作阳笥对照，同时与聚合酶链反应－序列特异性引物（ＰＣＲ－ＳＳＰ）检测方法作比较。结果：采用本方法
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| 关 键 词： | 人类白细胞抗原－A 等位基因 HLA－A PCR－SSOP |
Detection of HLA-A~*02 Alleles Using Polymerase Chain Reaction and Digoxigen-11-2-3 Dideoxyurdinctriphosphate Labeled Oligonucleotide Probes |
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| Zhai Ning,Hang Xiuping,He Weidong,Song Fangji.Detection of HLA-A~*02 Alleles Using Polymerase Chain Reaction and Digoxigen-11-2-3 Dideoxyurdinctriphosphate Labeled Oligonucleotide Probes[J].Journal of China Medical University,2000,29(3):215-216,219. |
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| Authors: | Zhai Ning Hang Xiuping He Weidong Song Fangji |
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| Abstract: | Objective:The experiment was designed to establish a method of polymerase chain reaction and sequence specific oligonucleotide probes (PCR SSOP) for identification HLA A2 alleles, and to analyze the practicability of the method. Methods: A PCR SSOP typing method, involving a single PCR amplification in conjunction with 23 digoxigenin labeled oligonucleotide probes, has been employed for the identification of 25 known HLA A *02 alleles. Twenty A2 ( ) samples in Shenyang were analyzed with this method and compared with PCR SSP typing method. Results: The finding of our method was same with those of PCR SSP. Conclusion:The method is highly specific and sensitive. The finding of this study has implications for the selection of donor in unrelated bone marrow transplantation and organ transplantation and human genetic studies. |
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| Keywords: | PCR SSOP HLA A *02 allele |
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