补肾中药对老龄大鼠睾丸功能影响的比较研究

目的：比较研究补肾中药六味地黄汤和八味地黄汤对老龄大鼠睾丸功能的影响。方法：8只3月龄成年雄性SD大鼠为正常对照组；24只22月龄老龄雄性SD大鼠随机分为3组，每组8只，分别为老龄组（SC）、六味地黄汤组（LW）、八味地黄汤组（BW）。LW组灌胃给药六味地黄汤，BW组灌胃给药八味地黄汤，连续用药60天。应用精子运动CASA分析和流式细胞仪检测技术，比较研究六味地黄汤和八味地黄汤对衰老大鼠精子运动能力、生殖细胞凋亡率和增殖指数的影响。结果：老龄组大鼠与成年组比较，精子密度明显下降（P〈0．05）；活动率、直线活动率、细胞凋亡率下降非常显著（P〈0．01）；细胞增殖指数明显增加（P〈0．05）。六味地黄汤和八味地黄汤对运动精子百分率有非常显著的提高（P〈0．01），生殖细胞凋亡率明显下降（P〈0．05，P〈0．01）。八味地黄汤组与六味地黄汤组比较，精子直线活动率明显增加（P〈0．05），生殖细胞凋亡率明显下降（P〈0．05）。结论：六味地黄汤和八味地黄汤在改善老龄大鼠睾丸功能方面，均有提高精子运动能力，减少其生殖细胞凋亡的作用。八味地黄汤在降低老龄大鼠睾丸生殖细胞凋亡率．提高精子直线活动率等方面优于六味地黄汤．

Comparative Study of Impact of Kidney Supplemented Chinese Medicinals on Orchic Function of Old Rats

Objective：to compare impact of kidney supplemented medicinals-Liuwei Dihuang and Bawei Dihuang on orchic function for old rats. Methods：Normal control group： eight 3-month SD adult rats （male） ; twenty-four 22-month old rats were randomly grouped to old age group, Liuwei treated group and Bawei treated group. Medicinals were intragastricly administrated for sixty consecutive days. Changes in sperm motility, generative cell apoptotic rate, and proliferation index were observed using semen auto-analysis （CASA） and flow cytometry technology. Results ：The old group showed a significant decline than the adult group in sperm concentration （P 〈 0. 05 ）, motile ratio, rectilinear movement ratio as well as apoptotic rate （ P 〈 0. 01 ）, and a rise of proliferation index （ P 〈 0. 05 ）. Both treated group are marked by elevated motile sperm ratio （ P 〈 0. 01 ） and dropped apoptotic rates （ respectively P 〈 0. 05, P 〈0. 01 ）. Bawei group was superior to Liuwei group in rise of rectilinear movement ratio and fall of apoptotic rate （ P 〈 0. 05 ）. Conclusion： Both medicinals were effective in raising sperm motility and reducing generative cell apoptotic rate for old rats in which aspects Bawei had advantage over Liuwei.