积雪草颗粒对单侧输尿管结扎大鼠肾组织结缔组织生长因子表达的影响

目的：研究积雪草颗粒（CTA）对单侧输尿管结扎（UUO）大鼠肾组织结缔组织生长因子（CTGF）表达的影响,为临床上应用CTA延缓肾衰竭的进展提供理论依据。方法：采用UUO大鼠模型,60只雄性SD大鼠随机分为6组：假手术（SOR）组、模型（UUO）组、积雪草颗粒大剂量组（CTAh）、积雪草颗粒中剂量组（CTAm）、积雪草颗粒小剂量组（CTAl）、蒙诺组（Monopril）。术后SOR、UUO组管饲与药物组同等容积的生理盐水;CTAh、CTAm、CTAl组于术后即每日分别管饲积雪草颗粒11.2mg.100g-1.d-1、5.6mg.100g-1.d-1、2.8mg.100g-1.d-1;Monopril组每日管饲蒙诺20mg.100g-1.d-1,术后21d处死各组大鼠。免疫组化法检测各肾组织CTGF的表达,RT-PCR方法检测各组CTGF mRNA含量。结果：UUO组CTGF的表达明显高于SOR组（P〈0.01）,而CTAh组和Monopril组各项指标明显低于UUO组（P〈0.01）,CTAh组和Monopril组无统计学差异（P〉0.05）,CTA组间比较大剂量组抑制CTGF表达优于中、小剂量组（P〈0.01）。结论：CTA减轻RIF的程度可能与下调CTGF的表达有关,且随剂量增加其抑制CTGF表达的生物学效应亦增大,呈量效依赖关系,CTA大剂量组抑制CTGF的效应与蒙诺组相似。

Effects of Centella Asiatica on Expression of Connective Colledge Tissue Growth Factor in UUO Rats

Objective：To invgestigate the expression of connective tissue growth factor （CTGF） in rats with renal tubulointerstital fibrosis （RIF）, and To provide a scientific evidence for the appliance of centella asiatica （CTA）. Methods：Unilateral ureteral obstruction （UUO） rat models were used. The expression and the concentration of CTGF in the obstructed kidneys was assessed by RT-PCR and immunohistochemistry staining methods respectively. Results： The level of CTGF in the UUO group increased remarkably compared with that in the sham-operated group （P （0.01）. Compared with the model group, the expression of CTGF was significantly decreased in both CTA group and Monopril group （P〈0.01） and there was no significant difference between the two groups（ P 〉0.05）. Conclusion： CTA may suppress the development of fibrosis by down - regulating the CTGF expression. With growing dose, the effect of CTA became more obvious. The case in CTAh group is similar to that in Monopril group in regulating the expression of CTGF.