细粒棘球蚴粗抗原的免疫纯化方法及其在免疫诊断中的应用

采用免疫过筛法将细粒棘球蚴囊液粗抗原（ＥｇＣＦ）和头节抗原（ＥｇＰ）与正常人混合血清中杂抗体结合而除去产生非特异反应的粗抗原部分，得到部分纯化的抗原ＥｇＣＦ２，ＥｇＰ２，纯化前后的抗原在酶联免疫吸附试验（ＥＬＩＳＡ）及斑点免疫结合试验（ＤＩＢＡ）中对４５例细粒棘球蚴病（ＣＥ）的诊断阳性率分别为：ＥｇＣＦ９３．３％，ＥｇＰ９５．６％，ＥｇＣＦ２９１．１％，ＥｇＰ２９３．３％，它们的敏感性无显差异。

A Method of Immunological Purification of Echinococcus granulosusAntigens and its Application in Immunodiagnosis of Echinococcosis

The partial pruified antigens of EgCF 2 and EgP 2 have been gained by using an immunofiltration method combained the crude antigens of EgCF and EgP with normal human sera to eliminate the cross reactive fractions. The dot immunoassay (DIBA) and enzyme linked immunosorbent assay (ELISA) have been performed with sera samples of 45 cases with cystic echincoccosis (CE), 22 cases of alveolar echinococcosis (AE), 10 cases with cysticercosis and 26 cases with other parasitic diseases. 32 normal sera were tested as control. The seropositive rates of crude antigens were 93.3%~95.6% in CE, 91.1%~93.3% in AE, 70%~80% in cysticercosis and 11.5% in other parasitic diseases. The false positive rates in controls were 11.1%~13.9%. But the seropositive rates with purified antigens EgCF 2 and EgP 2 were 91.1%~93.3% in CE, 81.8%~86.3% in AE, 60%~70% in oysticercosis and 3.8% in other parasitic diseases respectively. The false positive rate was 5.6% in the control. After purification, the specificity of the antigen has been obuiously increased and the nonspecific reaction reduced evidently.