食管癌和淋巴结转移组织中c-myc,hTERT和c-MET蛋白的表达

目的:探讨食管癌和淋巴结转移组织中c-myc、hTERT和c-MET的表达.方法:利用组织微阵列技术,采用免疫组化ABC法,测定58例食管癌及其相应的癌旁组织、8例转移淋巴结组织中c-myc、hTERT和c-MET的表达.结果:癌旁、癌及淋巴结转移组织中c-myc阳性率分别为7%,66%,75%;hTERT阳性率分别为0%,48%,75%;c-MET阳性率分别为7%,34%,75%;癌旁和癌组织间c-myc和hTERT的表达差异有统计学意义(P＜0.05),癌旁和癌组织间,以及癌和淋巴结转移灶之间c-MET的表达差异均有统计学意义(P＜0.05).食管癌组织中c-myc与hTERT的表达呈正相关(r=0.411,P=0.002),c-myc与c-MET的表达不相关(r=0.211,P=0.146).结论:c-myc、hTERT和c-MET的高表达可能与食管癌发生发展相关,c-myc可能参与了hTERT的激活过程,c-MET的高表达可能参与了食管癌淋巴结的转移过程.

Expression of c-myc, hTERT and c-MET in esophageal squamous cell carcinoma and lymph node metastatic tissue

Aim: To explore the role of c-myc, hTERT, and c-MET in esophageal squamous cell carcinoma (SCC) development and progression.Methods: A total of 58 cases of SCC, the corresponding adjacent normal epithelium, and 8 metastatic lymph node lesions were enrolled to detect the protein expression of c-myc, hTERT, and c-MET using tissue array technique and immunohistochemical ABC method. Results The positive expression rate of c-myc in SCC, adjacent lesions, and metastatic lymph node lesions were 66%, 7%, and 75%, respectively. The positive expression rates of hTERT were 0%, 48%, and 75% in adjacent lesion, cancer samples and metastatic lymph node lesions, respectively. There were evident differences in the expression rates of c-myc or hTERT between the adjacent lesion and cancer lesions (P<0.05). The positive rate of c-MET were 7%, 34% and 75% in adjacent lesion, cancer samples and metastatic lymph node lesions, respectively,and there was evident difference between adjacent lesions and cancer samples and between cancer samples and metastatic lymph node lesions (P<0.05). The expression of c-myc was positively correlated with that of hTERT(r=0.411,P=0.002), and was not correlated with that of c-MET(r=0.211,P=0.146) in SCC tissue. Conclusion: c-myc, hTERT and c-MET may be involved in SCC development and progression. c-myc may be involved in activating process for hTERT, and c-MET may be involved in lymph node metastasis in SCC.