双金痛立停胶囊对骨癌大鼠炎症因子表达及骨代谢的影响

目的 观察双金痛立停胶囊对骨癌痛大鼠炎症因子表达及骨代谢的影响.方法 采用Walker 256乳腺癌细胞复制胫骨癌痛大鼠模型,选取模型复制成功的大鼠随机分为模型组、双金痛立停胶囊组,另设假手术组.从术后第13天开始灌胃给药,每天1次,共10 d.末次给药后腹主动脉采血,运用酶联免疫法(ELISA)检测血前列腺素E2(PGE2)、白细胞介素6(IL-6)、Ⅰ型胶原交联羧基末端肽(ICTP)的含量.结果 与模型组比较,双金痛立停胶囊可明显降低骨癌痛大鼠血浆前列腺素E2、白细胞介素6和血清Ⅰ型胶原交联羧基末端肽的含量(P<0.05或P<0.01).结论 双金痛立停胶囊缓解大鼠骨癌痛可能与抑制炎症因子表达、抑制骨吸收调节骨代谢平衡有关.

Effects of Shuangjintongliting Capsule on inflammatory cytokines and bone metabolic markers in rats with bone cancer pain

Objective To study the effects of Shuangjintongliting Capsule on inflammatory cytokines and bone metabolic markers in rats with bone cancer pain.Methods The model of bone cancer pain in rats was established by injecting Walker 256 mammary gland carcinoma cells into tibial medullary cavity.The rats with bone cancer pain were randomly divided into model group and Shuangjintongliting Capsule group.At the same time,other rats with sham-operation were selected to be the normal control group.The contents of prostaglandin E2 (PGE2),interleukin-6 (IL-6) and collagen Ⅰ cross-linked carboxy terminal telopeptide(ICTP) were detected by enzyme linked immunosorbent assay(ELISA).Results Compared with the model group,Shuangjintongliting Capsule could markedly reduce the contents of PGE2,IL-6 and ICTP(P<0.05 or P<0.01).Conclusion Shuangjintongliting Capsule may inhibit the expression of inflammatory cytokines and bone resorption to alleviate bone cancer pain in rats.