低聚原花青素对大鼠肝纤维化氧化损伤的影响

目的 研究低聚原花青素抗肝纤维化的作用及机制.方法 SD大鼠随机分为正常对照组、模型组、扶正化瘀胶囊组、低聚原花青素低、中、高剂量组(60、120、240 mg·kg-1).采用腹腔注射四氯化碳(CCl4)构建肝纤维化模型,采用苏木精-伊红(HE)染色法进行肝脏病理学观察.生化检测血清丙氨酸转移酶(ALT)和天冬氨酸转移酶(AST),酶联免疫吸附(ELISA)法测定肝组织的超氧化物歧化酶(SOD)和丙二醛(MDA)含量.蛋白质免疫印迹法检测沉默信息调节因子1(SIRT1)和Nrf2蛋白表达.结果 低聚原花青素(60、120、240 mg·kg-1)组显著抑制四氯化碳诱导的大鼠肝纤维化程度,血清丙氨酸转移酶、天冬氨酸转移酶水平及肝脏组织中丙二醛水平明显降低,超氧化物歧化酶显著提高,并上调沉默信息调节因子1和Nrf2蛋白表达.结论 低聚原花青素具有抗纤维化的作用,其作用机制可能与其抗氧化作用并上调沉默信息调节因子1和Nrf2蛋白表达有关.

Effects of oligomeric proantho cyanidins on oxidative damage of rat liver fibrosis

Objective To study the effect and mechanism of oligomeric proantho cyanidins on hepatic fibrosis.Methods SD rats were divided randomly into 6 groups:control group,model group,Fuzheng Huayu Capsule group,oligomeric proantho cyanidins group(60,120,240 mg·kg-1).The hepatic fibrosis model was established by intraperitoneal injection of carbon tetrachloride(CCl4).Liver pathology was observed by hematoxylin-eosin (HE) staining.Biochemical method decect Serum alanine aminotransferase(ALT) and aspartate transferase(AST).The contents of superoxide dismutase(SOD) and malondialdehyde(MDA) in liver tissue were determined by enzyme-linked immunosorbent assay(ELISA).Western blot assay was used to detect Sirt1 and Nrf2 protein expression.Results Oligomeric proantho cyanidins(60,120,240 mg·kg-1)significantly inhibited CCl4-induced liver fibrosis in rats.The ALT and AST level in serum,MDA level in liver were significantly reduced,SOD significantly increased.Furthormore,Oligomeric proantho cyanidins were upregulated Sirt1 and Nrf2 protein expression.Conclusion Oligomeric proantho cyanidins have anti-fibrotic effects,its mechanism maybe related to its antioxidant effects.