RASSF2A基因甲基化在喉癌组织中的表达及意义

目的 探讨抑癌基因RASSF2A表达水平及其基因异常甲基化与喉癌的关系及意义。 方法 采用RT-PCR及甲基化特异性PCR(MSP)检测50例喉癌组织(实验组)和10例癌旁正常黏膜组织(对照组)中RASSF2A基因mRNA的表达水平及其启动子甲基化状态。 结果 喉癌组织中有54%(27/50)存在RASSF2A基因启动子,而对照组正常黏膜组织中未发现RASSF2A基因甲基化现象,差异有统计学意义(χ2=9.818, P=0.002)。RASSF2A甲基化水平程度与喉癌患者临床病理特征无明显相关性。喉癌组织中RASSF2A基因mRNA的表达水平明显低于正常黏膜组织(χ2=9.818, P=0.002)。RASSF2A基因甲基化的喉癌组织中其mRNA的表达减少,反之亦然。 结论 喉癌组织中RASSF2A基因CpG岛甲基化与其基因表达缺失有关,可能参与了喉癌的发生发展过程。

RASSF2A methylation in laryngeal squamous cell carcinoma

Objective To evaluate the effects of methylation on RASSF2A expression during the development of laryngeal squamous cell carcinoma (LSCC).Methods Methylation-specific PCR was used to measure RASSF2A methylation and mRNA levels in tissues from 50 LSCCs (LSCC group) and 10 normal laryngeal tissues (control group).Results Hypermethylation was detected in the promoter region of RASSF2A in 54％ (27/50) of the laryngeal cancer tissue samples but was not detected in control group tissues.The difference in hypermethylation was statistically significant (x2 =9.818,P =0.002).No significant correlations were found between the methylation levels of RASSF2A and the clinicopathological features of the patients with laryngeal cancer.The levels of RASSF2A mRNA in the LSCC group were significantly lower than those in normal mucosal tissues (x2 =9.818,P =0.002),because of methylation in RASSF2A in the laryngeal cancer tissue samples.Conclusion Epigenetic inactivation of RASSF2A through aberrant promoter methylation may play an important role during oncogenesis and the progression of LSCC.